Our QSS Assist™ TR-FRET kit includes assay buffer, Carna's protein kinase, and ATP/ULight™ labeled substrate peptide combined with metal cofactor. By following our Kit protocol, you can readily perform your kinase assay of interest and generate high quality data. The ATP concentration in the kit is fixed around the Km value determined for each kinase. The kit minimum order volume is 400dp. Please prepare the Eu-labeled antibody according to our recommendation.