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Mobility Shift Assay(MSA)/IMAP™ キナーゼアッセイ Preincubation試験ターゲット

強い阻害効果を示すキナーゼ阻害剤の中には、キナーゼへの結合が非常に遅いもの(Slow binder)もあることが知られています。Preincubation IC50値算出試験では、Slow binderに適切な実験手順を用い化合物本来の阻害活性を測定致します。室温での活性の安定性が確認されたキナーゼのみをターゲットとし、通常のMobility Shift Assayの試験条件に室温 30 分のキナーゼ+阻害剤での事前インキュベーション時間を加えて試験を行ないます。ATP濃度はKm値付近もしくは1mMからお選びください。

試験スケジュールおよびアッセイ詳細条件をご確認頂き、ご利用の際はPreincubation試験専用の申込書上でご希望ターゲットをご選択ください。ご記入済みの申込書はinfo@carnabio.com宛のメールに添付頂きご提出ください。
全キナーゼターゲット、セレクトパネルから選択をご希望の場合はこちらのページからお進みください。ご質問、ご要望などはお気軽にお問い合わせください。

Target Name Group ATP conc. Source
(Carna Cat. No.)
ABL(ABL1) Cytoplasmic Tyrosine Km bin./1mM 08-001
ABL(ABL1)[E255K] Cytoplasmic Tyrosine Km bin./1mM 08-094
ABL(ABL1)[T315I] Cytoplasmic Tyrosine Km bin./1mM 08-093
AKT1 Serine / Threonine Km bin./1mM 01-101
AKT2 Serine / Threonine Km bin. 01-102
AKT3 Serine / Threonine Km bin. 01-103
ALK Receptor Tyrosine Km bin./1mM 08-518
ALK[C1156Y] Receptor Tyrosine Km bin./1mM 08-530
ALK[F1174L] Receptor Tyrosine Km bin./1mM 08-519
ALK[G1202R] Receptor Tyrosine Km bin./1mM 08-544
ALK[G1269A] Receptor Tyrosine Km bin./1mM 08-537
ALK[L1196M] Receptor Tyrosine Km bin./1mM 08-529
ALK[R1275Q] Receptor Tyrosine Km bin./1mM 08-520
AMPKα1/β1/γ1 (PRKAA1/B1/G1) Serine / Threonine Km bin./1mM 02-113
AMPKα2/β1/γ1 Serine / Threonine Km bin. 02-114
ARG(ABL2) Cytoplasmic Tyrosine Km bin./1mM 08-102
AurA(AURKA)/TPX2 Serine / Threonine Km bin. 05-186
AurB(AURKB)/INCENP Serine / Threonine Km bin./1mM 05-102
BMX Cytoplasmic Tyrosine Km bin./1mM 08-179
BRSK1 Serine / Threonine Km bin./1mM 02-115
BRSK2 Serine / Threonine Km bin. 02-116
BUB1/BUB3 Serine / Threonine Km bin. 05-187
CaMK2α(CAMK2A) Serine / Threonine Km bin./1mM Km bin. 02-109
CaMK2β(CAMK2B) Serine / Threonine Km bin./1mM 02-110
CaMK2γ(CAMK2G) Serine / Threonine Km bin./1mM Km bin. 02-112
CaMK2δ(CAMK2D) Serine / Threonine Km bin./1mM Km bin. 02-111
CaMK4 Serine / Threonine Km bin./1mM 02-108
CDK1(CDC2)/CycB1 Serine / Threonine Km bin./1mM 04-102
CDK2/CycA2 Serine / Threonine Km bin./1mM 04-103
CDK2/CycE1 Serine / Threonine Km bin./1mM 04-165
CDK3/CycE1 Serine / Threonine Km bin. 04-104
CDK5/p25 Serine / Threonine Km bin./1mM 04-106
CGK2 Serine / Threonine Km bin. 01-143
CHK1(CHEK1) Serine / Threonine Km bin./1mM 02-117
CHK2(CHEK2) Serine / Threonine Km bin./1mM 02-162
CK1γ1 Serine / Threonine Km bin. 03-105
CK1γ2 Serine / Threonine Km bin. 03-106
CK1γ3 Serine / Threonine Km bin. 03-107
CK1δ Serine / Threonine Km bin./1mM 03-103
CK2α1/β (CSNK2A1) Serine / Threonine Km bin./1mM 05-184
CK2α2/β Serine / Threonine Km bin. 05-185
CLK1 Serine / Threonine Km bin./1mM 04-126
CLK2 Serine / Threonine Km bin./1mM 04-127
CLK3 Serine / Threonine Km bin. 04-128
DYRK1A Serine / Threonine Km bin./1mM 04-130
DYRK1B Serine / Threonine Km bin./1mM 04-131
DYRK2 Serine / Threonine Km bin./1mM 04-132
DYRK3 Serine / Threonine Km bin./1mM 04-133
EGFR (ERBB1) Receptor Tyrosine Km bin./1mM 08-115
EGFR[d746-750] (ERBB1) Receptor Tyrosine Km bin./1mM 08-527
EGFR[d746-750/C797S] NEW Receptor Tyrosine Km bin./1mM 08-564
EGFR[L858R] (ERBB1) Receptor Tyrosine Km bin./1mM 08-502
EGFR[L861Q] (ERBB1) Receptor Tyrosine Km bin./1mM 08-513
EGFR[T790M] (ERBB1) Receptor Tyrosine Km bin./1mM 08-194
EGFR[T790M/L858R] (ERBB1) Receptor Tyrosine Km bin./1mM 08-510
EPHA1 Receptor Tyrosine Km bin./1mM 08-119
EPHA2 Receptor Tyrosine Km bin./1mM 08-121
EPHA3 Receptor Tyrosine Km bin./1mM 08-122
EPHA4 Receptor Tyrosine Km bin./1mM 08-123
EPHA5 Receptor Tyrosine Km bin./1mM 08-124
EPHA6 Receptor Tyrosine Km bin./1mM 08-125
EPHA7 Receptor Tyrosine Km bin./1mM 08-126
EPHA8 Receptor Tyrosine Km bin./1mM 08-127
EPHB1 Receptor Tyrosine Km bin./1mM 08-128
EPHB2 Receptor Tyrosine Km bin./1mM 08-129
EPHB3 Receptor Tyrosine Km bin./1mM 08-130
EPHB4 Receptor Tyrosine Km bin./1mM 08-131
Erk1(MAPK3) Serine / Threonine Km bin./1mM 04-142
Erk2(MAPK1) Serine / Threonine Km bin./1mM 04-143
FER Cytoplasmic Tyrosine Km bin./1mM 08-139
FES Cytoplasmic Tyrosine Km bin./1mM 08-140
FGFR1 Receptor Tyrosine Km bin./1mM 08-133
FGFR2 Receptor Tyrosine Km bin./1mM 08-134
FGFR2[V564I] Receptor Tyrosine Km bin./1mM 08-546
FGFR4 Receptor Tyrosine Km bin./1mM 08-136
FGFR4[V550L] Receptor Tyrosine Km bin./1mM 08-526
FLT1 Receptor Tyrosine Km bin./1mM 08-189
FLT4 Receptor Tyrosine Km bin./1mM 08-190
FRK Cytoplasmic Tyrosine Km bin./1mM 08-167
GSK3α Serine / Threonine Km bin./1mM 04-140
GSK3β(GSK3B) Serine / Threonine Km bin./1mM 04-141
Haspin Serine / Threonine Km bin. 05-111
HER4 (ERBB4) Receptor Tyrosine Km bin./1mM 08-118
HIPK1 Serine / Threonine Km bin. 04-135
HIPK2 Serine / Threonine Km bin. 04-136
HIPK3 Serine / Threonine Km bin./1mM 04-137
IGF1R Receptor Tyrosine Km bin./1mM 08-141
IKKβ (IKBKB) Serine / Threonine Km bin./1mM 05-084
INSR Receptor Tyrosine Km bin./1mM 08-142
JAK2 Cytoplasmic Tyrosine Km bin./1mM 08-045
JNK1(MAPK8) Serine / Threonine Km bin./1mM 04-163
JNK2(MAPK9) Serine / Threonine Km bin./1mM 04-164
JNK3 (MAPK10) Serine / Threonine Km bin./1mM 04-150
KDR(VEGFR2) Receptor Tyrosine Km bin./1mM 08-191
LCK Cytoplasmic Tyrosine Km bin./1mM 08-170
LYNa Cytoplasmic Tyrosine Km bin./1mM 08-171
LYNb Cytoplasmic Tyrosine Km bin./1mM 08-172
MAPKAPK2 Serine / Threonine Km bin./1mM 02-142
MAPKAPK3 Serine / Threonine Km bin. 02-143
MAPKAPK5 Serine / Threonine Km bin. 02-144
MARK1 Serine / Threonine Km bin. 02-120
MARK2 Serine / Threonine Km bin. 02-121
MARK3 Serine / Threonine Km bin. 02-122
MARK4 Serine / Threonine Km bin./1mM 02-123
MER(MERTK) Receptor Tyrosine Km bin./1mM 08-108
MET Receptor Tyrosine Km bin./1mM 08-151
MET[D1228H] Receptor Tyrosine Km bin./1mM 08-540
MET[M1250T] Receptor Tyrosine Km bin./1mM 08-545
MET[Y1235D] Receptor Tyrosine Km bin./1mM 08-198
MNK1(MKNK1) Serine / Threonine Km bin. 02-145
MNK2 Serine / Threonine Km bin./1mM 02-146
MSK1 Serine / Threonine Km bin./1mM Km bin. 01-147
NEK2 Serine / Threonine Km bin./1mM 05-226
NEK4 Serine / Threonine Km bin. 05-128
NIM1K(MGC42105) Serine / Threonine Km bin. 02-175
NPM1-ALK Receptor Tyrosine Km bin./1mM 08-517
NuaK1(ARK5) Serine / Threonine Km bin./1mM 02-126
NuaK2 Serine / Threonine Km bin./1mM 02-127
p38α(MAPK14) Serine / Threonine Km bin./1mM 04-152
p38β (MAPK11) Serine / Threonine Km bin./1mM 04-153
p38γ (MAPK12) Serine / Threonine Km bin./1mM 04-155
p38δ (MAPK13) Serine / Threonine Km bin./1mM 04-154
p70S6K(RPS6KB1) Serine / Threonine Km bin./1mM 01-154
p70S6Kβ(RPS6KB2) Serine / Threonine Km bin. 01-155
PAK1 Serine / Threonine Km bin./1mM 07-123
PAK2 Serine / Threonine Km bin./1mM 07-124
PAK5 Serine / Threonine Km bin./1mM 07-127
PDGFRα(PDGFRA) Receptor Tyrosine Km bin./1mM 08-157
PDGFRα(PDGFRA)[D842V] Receptor Tyrosine Km bin./1mM 08-506
PDGFRα(PDGFRA)[V561D] Receptor Tyrosine Km bin./1mM 08-507
PDGFRβ(PDGFRB) Receptor Tyrosine Km bin./1mM 08-158
PIM1 Serine / Threonine Km bin./1mM 02-054
PKCε(PRKCE) Serine / Threonine Km bin./1mM 01-136
PKD1 Serine / Threonine Km bin. 02-157
PKD2 (PRKD2) Serine / Threonine Km bin./1mM 02-158
PYK2(PTK2B) Cytoplasmic Tyrosine Km bin./1mM 08-138
QIK (SIK2) Serine / Threonine Km bin./1mM 02-129
RET Receptor Tyrosine Km bin./1mM 08-159
RET[G691S] Receptor Tyrosine Km bin./1mM 08-522
RET[M918T] Receptor Tyrosine Km bin./1mM 08-508
RET[S891A] Receptor Tyrosine Km bin./1mM 08-523
RET[Y791F] Receptor Tyrosine Km bin./1mM 08-521
RON (MST1R) Receptor Tyrosine Km bin./1mM 08-152
ROS(ROS1) Receptor Tyrosine Km bin./1mM 08-163
RSK3(RPS6KA2) Serine / Threonine Km bin./1mM 01-151
RSK4(RPS6KA6) Serine / Threonine Km bin./1mM 01-152
SGK Serine / Threonine Km bin./1mM 01-158
SGK3 Serine / Threonine Km bin. 01-160
skMLCK Serine / Threonine Km bin. 02-150
SPHK1 Lipid Km bin. 11-105
SRC Cytoplasmic Tyrosine Km bin./1mM 08-173
SRM (SRMS) Cytoplasmic Tyrosine Km bin./1mM 08-174
TBK1 Serine / Threonine Km bin./1mM Km bin. 05-115
TEC Cytoplasmic Tyrosine Km bin./1mM 08-182
TIE2(TEK) Receptor Tyrosine Km bin./1mM 08-185
TRKB(NTRK2) Receptor Tyrosine Km bin./1mM 08-187
TRKC(NTRK3) Receptor Tyrosine Km bin./1mM 08-197
TSSK1 Serine / Threonine Km bin./1mM 02-364
TYRO3 Receptor Tyrosine Km bin./1mM 08-109
YES(YES1) Cytoplasmic Tyrosine Km bin./1mM 08-175
YES(YES1)[T348I] Cytoplasmic Tyrosine Km bin./1mM 08-533
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Preincubation IC50値算出試験

Preincubation 試験の手順

ご参考実験データ

Preincubationの実施によるIC50値の比較
Preincubationの有無によるIC50値の変化

アッセイスケジュール

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被験物質 受付締切日
MSA / IMAPTMアッセイ開始日
ADP-Glo™, MSA(Preincubation)アッセイ開始日

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