Carna Biosciences, Inc.

Products Search

Cell-based Tyrosine Kinase Assay Services

Carna Biosciences offers profiling services using a unique cell-based tyrosine kinase assay panel developed by Advanced Cellular Dynamics (ACD, Seattle, WA, USA). This service provides tyrosine kinase profiling and screening using transfectants in which activated recombinant kinase are expressed, and may help you to discover direct inhibitors to target tyrosine kinases.

Assay Services
  1. IC50 Determination : ten (10) serial concentrations in half-log dilutions from the starting concentration are tested in duplicate
  2. % Inhibition Study : three (3) serial concentrations in full-log dilutions from the starting concentrations are tested in duplicate

In addition to screening/profiling services, provision of cell lines on the list below is also avialable. For detailed provision conditions, please contact us.

Tyrosine Kinases

ABL (BCR-ABL)
ALK
ARG (ABL2)
AXL
BLK
BMX
CCK4 (PTK7)
DDR2
EGFR
EGFR[D746-750]
EGFR [L858R]
EGFR[L858R+T790M]
EGFR[L858R+C797S]
EGFR[L858R+T790M+C797S]
EGFR[L861Q]
EGFR[L861Q+T790M]
EGFR [T790M]
EphA1
EphA3
EphA4
EphA5
EphB1
EphB2
EphB4
FGFR1
FGFR1 [V561M]
FGFR2
FGFR2 [K660E]
FGFR2 [K660N]
FGFR2 [N550K]
FGFR2 [V565I]
FGFR2/AFF3
FGFR2/BICC1
FGFR2/CASP7
FGFR2/CCDC6
FGFR3
FGFR3 [K650M]
FGFR3/BAIAP2L1
FGFR4
FGFR4 [V550E]
FGR
FLT1(VEGFR1)
FLT3
FLT3-ITD
FLT3-ITD [D835V]
FLT3-ITD [D835Y]
FLT3-ITD [F691L]
FLT3-ITD [Y842C]
FLT3-ITD [Y842H]
FLT4(VEGFR3)
FRK
FYN
HCK
HER2(ERBB2)
HER3 (ERBB3)
IGF1R
INSR
ITK
KDR(VEGFR2)
KIT
KIT[D816V]
KIT[K642E]
KIT[N822H]
KIT[T670I]
KIT[V654A]
LCK
LYN
MER (MERTK)
MET
PDGFRα
PDGFRβ
RET
RET [V804M]
RON (MST1R)
ROR1
RYK
SRC
SYK
TIE1
TIE2
TRKA (NTRK1)
TRKB (NTRK2)
TRKC (NTRK3)
TYRO3
ZAP70

Principle & Method of ACD Cell-based Assays

The assay principle is based on the methods reportedby Daley & Baltimore (1988)* and Jonathan S.Melnick et al.(2005)**. In this cell-based assay, IL3-dependent Ba/F3 cells are transformed by transfection of an human recombinant kinase gene and in turn the modified cells are dependent on the activity of the recombinant kinase for survival and proliferation.

* Daley & Baltimore; Proc. Natl. Acad. Sci. U S A. 1988; 85(23):9312-6
** Melnick et al., pnas.0511292103

Principle & Method of ACD Cell-based Assays
ACD
About ACD
Advanced Cellular Dynamics (Seattle, WA, USA) is a leading provider of cell-based assay panel technologies and services to the life-sciences community. ACD develops and deploys families of cell-based screening assays, encompassing broad representations of important target gene families. Their assays are designed to simplify high-throughput screening and profiling of chemical entities in a physiologically relevant cellular environment.
LinkedIn