Live-cell Kinase Assays
For more details of method, please click HERE.
2 Detemination Studies
- IC50 Determination : ten (10) serial concentrations in half-log dilutions from the starting concentration are tested in duplicate
- % Inhibition Study : three (3) serial concentrations in full-log dilutions from the starting concentrations are tested in duplicate
ACD's Tyrosine Kinase Cell-Based Assay
Largest Commercially Available 94 Tyrosine Kinase Cell-Based Panel
FMS(CSF1R) has been withdrawn from the list for the moment.
|94 Tyrosine Kinases|
|ABL (BCR-ABL)||EphA1||FGFR3||HER3 (ERBB3)||PDGFRα|
|BLK||EphB1||FGFR4 [V550E]||JAK1||RON (MST1R)|
|BTK||EphB4||FLT1(VEGFR1)||JAK2 [V617F]||ROS (ROS1)|
|EGFR||FGFR1 [V561M]||FLT3-ITD [D835V]||KIT||SYK|
|FGFR2 [K660E]||FLT3-ITD [F691L]||KIT[K642E]||TIE2|
|EGFR [L858R]||FGFR2 [K660N]||FLT3-ITD [Y842C]||KIT[N822H]||TRKA (NTRK1)|
|FGFR2 [N550K]||FLT3-ITD [Y842H]||KIT[T670I]||TRKB (NTRK2)|
|EGFR[L858R+C797S]||FGFR2 [V565I]||FLT4(VEGFR3)||KIT[V654A]||TRKC (NTRK3)|
The assay principle is based on the methods reportedby Daley & Baltimore (1988)* and Jonathan S.Melnick et al.(2005)**. In this cell-based assay, IL3-dependent Ba/F3 cells are transformed by transfection of an human recombinant kinase gene and in turn the modified cells are dependent on the activity of the recombinant kinase for survival and proliferation.
Please select your target from above 94 tyrosine kinases. The % inhibition or IC50 value determination studies will be performed with your compounds. The validation study with the clinically-relevant kinase inhibitors reveals that this profiling results are consisted with those reported by others.
* Daley and Baltimore; Proc. Natl. Acad. Sci. USA. 1988; 85(23):9312-6
** Melnick et al., pnas.0511292103
Advanced Cellular Dynamics (Seattle, WA, USA) is a leading provider of cell-based assay panel technologies and services to the life-sciences community. ACD develops and deploys families of cell-based screening assays, encompassing broad representations of important target gene families. Their assays are designed to simplify high-throughput screening and profiling of chemical entities in a physiologically relevant cellular environment.