Products & Services

Live-cell Kinase Assays

Carna Biosciences offers the profiling service using an unique cell-based tyrosine kinase assay panel developed by Advanced Cellular Dynamics (Seattle, CA USA). This service provides tyrosine kinase profiling and screening using transfectants in which activated recombinant kinase are expressed and may help you to discover direct inhibitors to target tyrosine kinases.
For more details of method, please click HERE.
2 Detemination Studies
  1. IC50 Determination : ten (10) serial concentrations in half-log dilutions from the starting concentration are tested in duplicate
  2. % Inhibition Study : three (3) serial concentations in full-log dilutions from the starting concentrations are tested in duplicate
ACD's Tyrosine Kinase Cell-Based Assay

Largest Commercially Available 93 Tyrosine Kinase Cell-Based Panel

93 Tyrosine Kinases
ABL (BCR-ABL) EphA1 FGFR3 HER2(ERBB2) PDGFRβ
ALK EphA3 FGFR3 [K650M] HER3 (ERBB3) RET
ARG (ABL2) EphA4 FGFR3/BAIAP2L1 IGF1R RET [V804M]
AXL EphA5 FGFR4 INSR RON (MST1R)
BLK EphB1 FGFR4 [V550E] JAK1 ROR1
BMX EphB2 FGR JAK2 ROS (ROS1)
BTK EphB4 FLT1(VEGFR1) JAK3 RYK
CCK4 (PTK7) FAK FLT3 KDR(VEGFR2) SRC
DDR2 FGFR1 FLT3-ITD KIT SYK
EGFR FGFR1 [V561M] FLT3-ITD [D835V] KIT[D816V] TIE1
EGFR[D746-750] FGFR2 FLT3-ITD [D835Y] KIT[K642E] TIE2
EGFR
[D746-750+T790M]
FGFR2 [K660E] FLT3-ITD [F691L] KIT[N822H] TRKA (NTRK1)
EGFR [L858R] FGFR2 [K660N] FLT3-ITD [Y842C] KIT[T670I] TRKB (NTRK2)
EGFR
[L858R+T790M]
FGFR2 [N550K] FLT3-ITD [Y842H] KIT[V654A] TRKC (NTRK3)
EGFR[L858R+C797S] FGFR2 [V565I] FLT4(VEGFR3) LCK TYK2
EGFR
[L858R+T790M+C797S]
FGFR2/AFF3 FMS (CSF1R) LYN TYRO3
EGFR[L861Q] FGFR2/BICC1 FRK MER (MERTK) ZAP70
EGFR
[L861Q+T790M]
FGFR2/CASP7 FYN MET  
EGFR [T790M] FGFR2/CCDC6 HCK PDGFRα  

The assay principle is based on the methods reportedby Daley & Baltimore (1988)* and Jonathan S.Melnick et al.(2005)**. In this cell-based assay, IL3-dependent Ba/F3 cells are transformed by transfection of an human recombinant kinase gene and in turn the modified cells are dependent on the activity of the recombinant kinase for survival and proliferation.

Please select your target from above 93 tyrosine kinases. The % inhibition or IC50 value determination studies will be performed with your compounds. The validation study with the clinically-relevant kinase inhibitors reveals that this profiling results are consisted with those reported by others.

* Daley and Baltimore; Proc. Natl. Acad. Sci. USA. 1988; 85(23):9312-6
** Melnick et al., pnas.0511292103

About ACD

Advanced Cellular Dynamics (Seattle, CA USA) is a leading provider of cell-based assay panel technologies and services to the life-sciences community. ACD develops and deploys families of cell-based screening assays, encompassing broad representations of important target gene families. Their assays are designed to simplify high-throughput screening and profiling of chemical entities in a physiologically relevant cellular environment.
http://advancedcelldynamics.com/

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