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HTRF™/LanthaScreen™キナーゼアッセイサポート

HTRF™（Revvity社）およびLanthaScreen™（ThermoFisher Scientific社）キナーゼアッセイは時間分解蛍光共鳴エネルギー移動（TR-FRET）技術を利用しキナーゼと化合物のアフィニティを測定するアッセイです。均一な反応系で実施される為スループットが高く薬剤スクリーニングにも最適です。

カルナのビオチン化キナーゼタンパク質を用いたTR-FRETアッセイでは抗体を必要としない為、非常に簡便に幅広いターゲットでのキナーゼアッセイの構築・再現性の高いデータの取得が可能となります。お客様がHTRF™およびLanthaScreen™キナーゼアッセイを構築し薬剤評価やスクリーニングを行う際のご参考用に、カルナでは自社タンパク質製品を用いて測定したHTRF™/LanthaScreen™キナーゼアッセイ実施条件を公開しております。

このページで紹介されているアッセイ条件で取得されたデータはAffinity Data欄添付のPDFでご確認頂けます。一覧表にあるビオチン化キナーゼタンパク質製品、アッセイバッファー製品は全て当社でご購入可能です。

リストにある当社製品についてのお問い合わせ、ご相談等はこちらより、またはinfo@carnabio.com からご連絡ください。

HTRF™/LanthaScreen™キナーゼアッセイ　反応条件一覧表

Kinase Name Protein Kinase Tracer
（各提供メーカーよりご購入下さい）		 Assay
Buffer		 Sample
Affinity
Data
Tag Carna Product Name Cat. No. Product Name Cat. No. Manufacturer Cat. No.
ABL(ABL1) BTN BTN-ABL(ABL1)
08-401-20N Dasatinib-Red 62KB02REDC Revvity ASBF01
ABL(ABL1)[T315I] BTN BTN-ABL(ABL1)[T315I]
08-493-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
BTN BTN-ABL(ABL1)[T315I]
08-493-20N Dasatinib-Red 62KB02REDC Revvity ASBF01
ACTR2B(ACVR2B) BTN BTN-ACTR2B(ACVR2B)
09-432-20N Kinase Tracer 178 PV5593 Thermo Fisher Scientific ASBF01
AKT1 BTN BTN-AKT1
01-401-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
AKT2 BTN BTN-AKT2
01-402-20N Kinase Tracer 236 PV5592 Thermo Fisher Scientific ASBF01
ALK BTN BTN-ALK
08-405-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
ALK[C1156Y] BTN BTN-ALK[C1156Y]
08-730-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
ALK[F1174L] BTN BTN-ALK[F1174L]
08-719-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
ALK[F1174L／G1202R] BTN BTN-ALK[F1174L／G1202R]
08-768-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
ALK[G1202R] BTN BTN-ALK[G1202R]
08-744-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
ALK[G1269A] BTN BTN-ALK[G1269A]
08-737-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
ALK[I1171N] BTN BTN-ALK[I1171N]
08-766-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
ALK[I1171N／L1198F] BTN BTN-ALK[I1171N／L1198F]
08-767-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
ALK[I1171S] BTN BTN-ALK[I1171S]
08-769-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
ALK[L1196M] BTN BTN-ALK[L1196M]
08-429-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
ALK[L1196M／G1202R] BTN BTN-ALK[L1196M／G1202R]
08-756-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
ALK[L1198F] BTN BTN-ALK[L1198F]
08-771-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
ALK[R1275Q] BTN BTN-ALK[R1275Q]
08-720-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
ALK[V1180L] BTN BTN-ALK[V1180L]
08-770-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
AMPKα1/β1/γ1 BTN BTN-AMPKα1/β1/γ1
02-413-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
AMPKα1/β2/γ1 BTN BTN-AMPKα1/β2/γ1
02-447-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
AurA BTN BTN-AurA
05-401-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
AurB(AURKB)／INCENP BTN BTN-AurB(AURKB)／INCENP
05-402-21N Staurosporine-Red 62KB01REDC Revvity ASBF01
AXL BTN BTN-AXL
08-407-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
BARK1(ADRBK1) BTN BTN-BARK1(ADRBK1)
01-411-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
BMX BTN BTN-BMX
08-479-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
BRAF BTN BTN-BRAF
09-422-20N Kinase Tracer 178 PV5593 Thermo Fisher Scientific ASBF01
BRK(PTK6) BTN BTN-BRK(PTK6)
08-465-20N Dasatinib-Red 62KB02REDC Revvity ASBF01
BTN BTN-BRK(PTK6)[non-activated]
08-465-23N Dasatinib-Red 62KB02REDC Revvity ASBF01
BTK BTN BTN-BTK
08-480-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
BTN BTN-BTK[non-activated]
08-480-23N Staurosporine-Red 62KB01REDC Revvity ASBF01
BTK[C481S] BTN BTN-BTK[C481S]
08-417-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
BTN BTN-BTK[C481S][non-activated]
08-417-23N Staurosporine-Red 62KB01REDC Revvity ASBF01
BTK[M437R] BTN BTN-BTK[M437R]
08-705-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
BTN BTN-BTK[M437R][non-activated]
08-705-23N Staurosporine-Red 62KB01REDC Revvity ASBF01
BTK[T316A] BTN BTN-BTK[T316A]
08-418-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
BTN BTN-BTK[T316A][non-activated]
08-418-23N Staurosporine-Red 62KB01REDC Revvity ASBF01
BTK[T474I] BTN BTN-BTK[T474I]
08-419-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
BTN BTN-BTK[T474I][non-activated]
08-419-23N Staurosporine-Red 62KB01REDC Revvity ASBF01
BTK[T474S] BTN BTN-BTK[T474S]
08-420-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
BTN BTN-BTK[T474S][non-activated]
08-420-23N Staurosporine-Red 62KB01REDC Revvity ASBF01
BTK[V416L] BTN BTN-BTK[V416L]
08-706-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
BTN BTN-BTK[V416L][non-activated]
08-706-23N Staurosporine-Red 62KB01REDC Revvity ASBF01
CaMK2β(CAMK2B) BTN BTN-CaMK2β(CAMK2B)
02-410-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
CaMK2δ(CAMK2D) BTN BTN-CaMK2δ(CAMK2D)
02-411-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
CDK10(PISSLRE)／CycQ BTN BTN-CDK10(PISSLRE)／CycQ
04-471-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
CDK19(CDC2L6) BTN BTN-CDK19(CDC2L6)
04-730-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
CDK19(CDC2L6)／CycC BTN BTN-CDK19(CDC2L6)／CycC
04-412-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
CDK2／CycA2 BTN BTN-CDK2／CycA2
04-403-20N Kinase Tracer 222 PV6121 Thermo Fisher Scientific ASBF01
BTN BTN-CDK2／CycA2
04-403-20N Kinase Tracer 314 PV6087 Thermo Fisher Scientific ASBF01
CDK5／p35 BTN BTN-CDK5／p35
04-492-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
CDK8 BTN BTN-CDK8
04-496-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
CDK8／CycC BTN BTN-CDK8／CycC
04-409-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
CDK9／CycK BTN BTN-CDK9／CycK
04-411-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
CDK9／CycT1 BTN BTN-CDK9／CycT1
04-410-20N Kinase Tracer 236 PV5592 Thermo Fisher Scientific ASBF01
CDK9／CycT2 BTN BTN-CDK9／CycT2
04-421-20N Kinase Tracer 236 PV5592 Thermo Fisher Scientific ASBF01
CLK4 BTN BTN-CLK4
04-429-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
DAPK3 BTN BTN-DAPK3
02-436-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
DDR1 BTN BTN-DDR1
08-432-20N Dasatinib-Red 62KB02REDC Revvity ASBF01
DDR1(SRC treated) BTN BTN-DDR1(SRC treated)
08-732-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
DDR2 BTN BTN-DDR2
08-414-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
DYRK1A BTN BTN-DYRK1A
04-430-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
EGFR BTN BTN-EGFR
08-415-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
BTN BTN-EGFR
08-415-20N Dasatinib-Red 62KB02REDC Revvity ASBF01
EGFR[C797S] BTN BTN-EGFR[C797S]
08-761-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
BTN BTN-EGFR[C797S]
08-761-20N Dasatinib-Red 62KB02REDC Revvity ASBF01
EGFR[C797S／L858R] BTN BTN-EGFR[C797S／L858R]
08-763-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
BTN BTN-EGFR[C797S／L858R]
08-763-20N Dasatinib-Red 62KB02REDC Revvity ASBF01
EGFR[d746-750] BTN BTN-EGFR[d746-750]
08-427-20N Dasatinib-Red 62KB02REDC Revvity ASBF01
EGFR[d746-750／C797S] BTN BTN-EGFR[d746-750／C797S]
08-764-20N Dasatinib-Red 62KB02REDC Revvity ASBF01
EGFR[L858R] BTN BTN-EGFR[L858R]
08-402-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
BTN BTN-EGFR[L858R]
08-402-20N Dasatinib-Red 62KB02REDC Revvity ASBF01
EGFR[T790M] BTN BTN-EGFR[T790M]
08-494-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
BTN BTN-EGFR[T790M]
08-494-20N Dasatinib-Red 62KB02REDC Revvity ASBF01
EGFR[T790M／C797S／L858R] BTN BTN-EGFR[T790M／C797S／L858R]
08-759-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
EGFR[T790M／L858R] BTN BTN-EGFR[T790M／L858R]
08-410-20N Dasatinib-Red 62KB02REDC Revvity ASBF01
BTN BTN-EGFR[T790M／L858R]
08-410-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
EPHA2 BTN BTN-EPHA2
08-421-20N Dasatinib-Red 62KB02REDC Revvity ASBF01
BTN BTN-EPHA2[non-activated]
08-421-23N Dasatinib-Red 62KB02REDC Revvity ASBF01
FAK(PTK2) BTN BTN-FAK(PTK2)
08-437-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
FGFR1 BTN BTN-FGFR1
08-435-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
BTN BTN-FGFR1[non-activated]
08-435-23N Staurosporine-Red 62KB01REDC Revvity ASBF01
FGFR2 BTN BTN-FGFR2
08-434-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
BTN BTN-FGFR2[non-activated]
08-434-23N Staurosporine-Red 62KB01REDC Revvity ASBF01
FGFR2[C491A／V564L] BTN BTN-FGFR2[C491A／V564L]
08-906-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
BTN BTN-FGFR2[C491A／V564L][non-activated]
08-906-23N Staurosporine-Red 62KB01REDC Revvity ASBF01
FGFR2[E565A] BTN BTN-FGFR2[E565A]
08-907-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
BTN BTN-FGFR2[E565A][non-activated]
08-907-23N Staurosporine-Red 62KB01REDC Revvity ASBF01
FGFR2[K659M] BTN BTN-FGFR2[K659M]
08-927-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
BTN BTN-FGFR2[K659M][non-activated]
08-927-23N Staurosporine-Red 62KB01REDC Revvity ASBF01
FGFR2[L617V] BTN BTN-FGFR2[L617V]
08-926-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
BTN BTN-FGFR2[L617V][non-activated]
08-926-23N Staurosporine-Red 62KB01REDC Revvity ASBF01
FGFR2[M537I] BTN BTN-FGFR2[M537I]
08-922-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
BTN BTN-FGFR2[M537I][non-activated]
08-922-23N Staurosporine-Red 62KB01REDC Revvity ASBF01
FGFR2[M537I／M538L] BTN BTN-FGFR2[M537I／M538L]
08-924-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
BTN BTN-FGFR2[M537I／M538L][non-activated]
08-924-23N Staurosporine-Red 62KB01REDC Revvity ASBF01
FGFR2[M538L] BTN BTN-FGFR2[M538L]
08-923-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
BTN BTN-FGFR2[M538L][non-activated]
08-923-23N Staurosporine-Red 62KB01REDC Revvity ASBF01
FGFR2[N549D] BTN BTN-FGFR2[N549D]
08-908-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
BTN BTN-FGFR2[N549D][non-activated]
08-908-23N Staurosporine-Red 62KB01REDC Revvity ASBF01
FGFR2[N549H] BTN BTN-FGFR2[N549H]
08-901-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
BTN BTN-FGFR2[N549H][non-activated]
08-901-23N Staurosporine-Red 62KB01REDC Revvity ASBF01
FGFR2[N549K] BTN BTN-FGFR2[N549K]
08-909-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
BTN BTN-FGFR2[N549K][non-activated]
08-909-23N Staurosporine-Red 62KB01REDC Revvity ASBF01
FGFR2[N549K／V564F] BTN BTN-FGFR2[N549K／V564F]
08-925-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
BTN BTN-FGFR2[N549K／V564F][non-activated]
08-925-23N Staurosporine-Red 62KB01REDC Revvity ASBF01
FGFR2[V564F] BTN BTN-FGFR2[V564F]
08-910-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
BTN BTN-FGFR2[V564F][non-activated]
08-910-23N Staurosporine-Red 62KB01REDC Revvity ASBF01
FGFR2[V564I] BTN BTN-FGFR2[V564I]
08-902-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
BTN BTN-FGFR2[V564I][non-activated]
08-902-23N Staurosporine-Red 62KB01REDC Revvity ASBF01
FGFR2[V564L] BTN BTN-FGFR2[V564L]
08-904-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
BTN BTN-FGFR2[V564L][non-activated]
08-904-23N Staurosporine-Red 62KB01REDC Revvity ASBF01
FGFR3 BTN BTN-FGFR3
08-433-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
FGFR4 BTN BTN-FGFR4
08-436-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
FLT3 BTN BTN-FLT3
08-454-21N Staurosporine-Red 62KB01REDC Revvity ASBF01
BTN BTN-FLT3[non-activated]
08-454-23N Staurosporine-Red 62KB01REDC Revvity ASBF01
FMS(CSF1R) BTN BTN-FMS(CSF1R)
08-455-21N Staurosporine-Red 62KB01REDC Revvity ASBF01
BTN BTN-FMS(CSF1R)[non-activated]
08-455-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
FYN [isoform a] BTN BTN-FYN [isoform a]
08-468-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
GSK3β(GSK3B) BTN BTN-GSK3β(GSK3B)
04-441-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
IGF1R BTN BTN-IGF1R
08-441-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
IKKα(CHUK) BTN BTN-IKKα(CHUK)
05-412-20N Kinase Tracer 236 PV5592 Thermo Fisher Scientific ASBF01
IKKβ BTN BTN-IKKβ
05-484-20N Kinase Tracer 236 PV5592 Thermo Fisher Scientific ASBF01
INSR BTN BTN-INSR
08-442-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
IRAK4 BTN BTN-IRAK4
09-445-20N Kinase Tracer 222 PV6121 Thermo Fisher Scientific ASBF01
ITK BTN BTN-ITK
08-481-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
JAK2 BTN BTN-JAK2
08-445-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
KDR BTN BTN-KDR
08-491-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
KIT BTN BTN-KIT
08-456-21N Staurosporine-Red 62KB01REDC Revvity ASBF01
BTN BTN-KIT
08-456-21N Sunitinib-Red 62KB03REDC Revvity ASBF01
BTN BTN-KIT[non-activated]
08-456-23N Sunitinib-Red 62KB03REDC Revvity ASBF01
LATS2／MOBKL1A BTN BTN-LATS2／MOBKL1A
01-424-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
LCK BTN BTN-LCK
08-470-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
LIMK2 BTN BTN-LIMK2
09-406-20N Dasatinib-Red 62KB02REDC Revvity ASBF01
LYNa BTN BTN-LYNa
08-471-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
MAP2K1[inactive] BTN BTN-MAP2K1[inactive]
07-441-10-20N Kinase Tracer 236 PV5592 Thermo Fisher Scientific ASBF01
MER(MERTK) BTN BTN-MER(MERTK)
08-408-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
MET BTN BTN-MET
08-451-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
MET[D1228H] BTN BTN-MET[D1228H]
08-440-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
MET[Y1230C] BTN BTN-MET[Y1230C]
08-709-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
MLK1(MAP3K9) BTN BTN-MLK1(MAP3K9)
09-415-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
MLK2(MAP3K10) BTN BTN-MLK2(MAP3K10)
09-416-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
MLK3(MAP3K11) BTN BTN-MLK3(MAP3K11)
09-417-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
MST2(STK3) BTN BTN-MST2(STK3)
07-417-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
NEK9 BTN BTN-NEK9
05-433-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
NuaK2 BTN BTN-NuaK2
02-427-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
p38α(MAPK14) BTN BTN-p38α(MAPK14)
04-452-20N Kinase Tracer 199 PV5830 Thermo Fisher Scientific ASBF01
p38α(MAPK14)[inactive] BTN BTN-p38α(MAPK14)[inactive]
04-452-10-20N Kinase Tracer 199 PV5830 Thermo Fisher Scientific ASBF01
PAK4 BTN BTN-PAK4
07-426-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
PAK6 BTN BTN-PAK6
07-428-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
PDGFRα(PDGFRA) BTN BTN-PDGFRα(PDGFRA)[non-activated]
08-457-23N Dasatinib-Red 62KB02REDC Revvity ASBF01
PDGFRβ(PDGFRB) BTN BTN-PDGFRβ(PDGFRB)
08-458-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
BTN BTN-PDGFRβ(PDGFRB)[non-activated]
08-458-23N Sunitinib-Red 62KB03REDC Revvity ASBF01
PIK3CA[E542K]／PIK3R1 BTN BTN-PIK3CA[E542K]／PIK3R1
11-413-20N Kinase Tracer 1710 PV6088 Thermo Fisher Scientific ASBF01
PIK3CA[E545K]／PIK3R1 BTN BTN-PIK3CA[E545K]／PIK3R1
11-414-20N Kinase Tracer 1710 PV6088 Thermo Fisher Scientific ASBF01
PIK3CA[H1047R]／PIK3R1 BTN BTN-PIK3CA[H1047R]／PIK3R1
11-415-20N Kinase Tracer 1710 PV6088 Thermo Fisher Scientific ASBF01
PIK3CA[P539R]／PIK3R1 BTN BTN-PIK3CA[P539R]／PIK3R1
11-412-20N Kinase Tracer 1710 PV6088 Thermo Fisher Scientific ASBF01
PIK3CA[R88Q]／PIK3R1 BTN BTN-PIK3CA[R88Q]／PIK3R1
11-411-20N Kinase Tracer 1710 PV6088 Thermo Fisher Scientific ASBF01
PIK3CA／PIK3R1 BTN BTN-PIK3CA／PIK3R1
11-401-20N Kinase Tracer 1710 PV6088 Thermo Fisher Scientific ASBF01
PIK3CB／PIK3R1 BTN BTN-PIK3CB／PIK3R1
11-402-20N Kinase Tracer 314 PV6087 Thermo Fisher Scientific ASBF01
PIK3CD／PIK3R1 BTN BTN-PIK3CD／PIK3R1
11-403-20N Kinase Tracer 1710 PV6088 Thermo Fisher Scientific ASBF01
BTN BTN-PIK3CD／PIK3R1
11-403-20N Kinase Tracer 314 PV6087 Thermo Fisher Scientific ASBF01
PIK3CG BTN BTN-PIK3CG
11-404-20N Kinase Tracer 1710 PV6088 Thermo Fisher Scientific ASBF01
BTN BTN-PIK3CG
11-404-20N Kinase Tracer 314 PV6087 Thermo Fisher Scientific ASBF01
PIKFYVE(PIP5K3) BTN BTN-PIKFYVE(PIP5K3)
11-418-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
PKACα(PRKACA) BTN BTN-PKACα(PRKACA)
01-427-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
PKCα(PRKCA) BTN BTN-PKCα(PRKCA)
01-433-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
PKCη BTN BTN-PKCη
01-438-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
PKN1 BTN BTN-PKN1
01-444-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
PYK2(PTK2B) BTN BTN-PYK2(PTK2B)
08-438-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
RAF1 BTN BTN-RAF1
09-425-20N Kinase Tracer 178 PV5593 Thermo Fisher Scientific ASBF01
RAF1(FL)[Y340E／Y341E] BTN BTN-RAF1(FL)[Y340E／Y341E]
09-427-20N Kinase Tracer 178 PV5593 Thermo Fisher Scientific ASBF01
RET BTN BTN-RET
08-459-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
BTN BTN-RET
08-459-20N Kinase Tracer 236 PV5592 Thermo Fisher Scientific ASBF01
BTN BTN-RET[non-activated]
08-459-23N Staurosporine-Red 62KB01REDC Revvity ASBF01
RET[V804M] BTN BTN-RET[V804M]
08-460-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
BTN BTN-RET[V804M]
08-460-20N Kinase Tracer 236 PV5592 Thermo Fisher Scientific ASBF01
BTN BTN-RET[V804M][non-activated]
08-460-23N Staurosporine-Red 62KB01REDC Revvity ASBF01
BTN BTN-RET[V804M][non-activated]
08-460-23N Kinase Tracer 236 PV5592 Thermo Fisher Scientific ASBF01
SRC BTN BTN-SRC
08-473-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
SYK BTN BTN-SYK
08-476-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
BTN BTN-SYK[non-activated]
08-476-23N Staurosporine-Red 62KB01REDC Revvity ASBF01
TAK1-TAB1(MAP3K7) BTN BTN-TAK1-TAB1(MAP3K7)
09-419-21C Staurosporine-Red 62KB01REDC Revvity ASBF01
TBK1 BTN BTN-TBK1
05-415-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
TEC BTN BTN-TEC
08-482-20N Dasatinib-Red 62KB02REDC Revvity ASBF01
BTN BTN-TEC[non-activated]
08-482-23N Dasatinib-Red 62KB02REDC Revvity ASBF01
TGFβR1(TGFBR1) BTN BTN-TGFβR1(TGFBR1)
09-441-20N Kinase Tracer 178 PV5593 Thermo Fisher Scientific ASBF01
TIE2(TEK) BTN BTN-TIE2(TEK)
08-485-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
TNIK BTN BTN-TNIK
07-438-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
TRKA(NTRK1) BTN BTN-TRKA(NTRK1)
08-486-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
BTN BTN-TRKA(NTRK1)[non-activated]
08-486-23N Staurosporine-Red 62KB01REDC Revvity ASBF01
TRKB(NTRK2) BTN BTN-TRKB(NTRK2)
08-487-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
BTN BTN-TRKB(NTRK2)[non-activated]
08-487-23N Staurosporine-Red 62KB01REDC Revvity ASBF01
TRKC(NTRK3) BTN BTN-TRKC(NTRK3)
08-497-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
TXK BTN BTN-TXK
08-483-20N Dasatinib-Red 62KB02REDC Revvity ASBF01
TYRO3 BTN BTN-TYRO3
08-409-20N Staurosporine-Red 62KB01REDC Revvity ASBF01
BTN BTN-TYRO3[non-activated]
08-409-23N Staurosporine-Red 62KB01REDC Revvity ASBF01
WEE1 BTN BTN-WEE1
05-477-20N Dasatinib-Red 62KB02REDC Revvity ASBF01
WNK4 BTN BTN-WNK4
05-482-20N Kinase Tracer 1710 PV6088 Thermo Fisher Scientific ASBF01
YES(YES1) BTN BTN-YES(YES1)
08-475-20N Staurosporine-Red 62KB01REDC Revvity ASBF01

当社 HTRF™/LanthaScreen™キナーゼアッセイプロトコール

  • アッセイバッファー（20 mM HEPES、0.01% Triton X-100、 2 mM DTT、pH 7.5）を調製。
  • アッセイバッファーを用いて4倍濃度のEu-Streptavidin溶液、4倍濃度のトレーサー溶液と各キナーゼ反応に必要な添加物を含む4倍濃度のキナーゼ/金属溶液を調製。
  • アッセイバッファー 5µL、4倍濃度のキナーゼ/金属溶液 5µL、4倍濃度のトレーサー溶液 5µL、および4倍濃度のEu-Streptavidin溶液 5µLをポリスチレン製384ウェル黒色マイクロプレート（Corning #3573）の各ウェルで混合し、室温で1時間インキュベート。
  • 各ウェルをTR-FRET(Ex 320nm, Em 665nm)で測定（EnVision 2104 (Revvity））し、キナーゼ活性をEmission ratio（665/615）で評価。
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