Our biotin-labeled peptide substrates, each labeled with a biotin molecule at the N-terminus (except for the Histone H3 peptide, which is labeled at the C-terminus), are optimized using our protein kinases for assays across various platforms, including ADP-Glo™ and HTRF™. These substrates are suitable for a wide range of research applications, such as high-throughput screening and protein–protein interaction studies.

If you are looking for suitable substrates for Carna's protein kinases, please contact us from here or at info@carnabio.com.
Note: Peptide substrate sequences are proprietary and not available for disclosure.

Substrates sold as:　100 μL at 2ｍM concentration

Catalog No.	Substrate Name	Kinases verified in Carna's assays (ADP-Glo™ assay platform)
BTSU01	Biotin-Srctide	ALK, ALK [C1156Y], ALK [F1174L], ALK [G1202R], ALK [G1269A], ALK [L1196M], ALK [R1275Q], ALK [T1151_L1152insT], BLK, BMX, BTK, BTK [C481S], CSK, EGFR, EGFR[C797S/L858R], EGFR [d746-750], EGFR[d746-750/C797S], EGFR [d746-750/T790M], EGFR[d746-750/T790M/C797S], EGFR [D770_N771insNPG], EGFR [L858R], EGFR [L861Q], EGFR [T790M], EGFR[T790M/C797S/L858R], EGFR [T790M/L858R], EML4-ALK, FER, FES, FGR, FLT3, FMS(CSF1R), FRK, FYN [isoform a], FYN [isoform b], HCK, HER2(ERBB2), HER4(ERBB4), ITK, JAK2, JAK3, KIT, KIT [D816E], KIT [D816V], KIT [D816Y], KIT [T670I], KIT [V560G], KIT [V654A], LCK, LTK, LYNa, LYNb, MET, MET [D1228H], MET [M1250T], MET [Y1235D], NPM1-ALK, RON(MST1R), SRC, TEC, TRKB(NTRK2), TRKC(NTRK3), TXK, TYK2, YES(YES1), YES(YES1) [T348I]
BTSU02	Biotin-CSKtide	AXL, FGFR1, FGFR1 [V561M], FGFR2, FGFR2 [V564I], FGFR3, FGFR3 [K650E], FGFR3 [K650M], FGFR3 [V555L], FGFR3 [V555M], FGFR4, FGFR4 [N535K], FGFR4 [V550E], FGFR4 [V550L], FLT1, FLT4, KDR, MER(MERTK), MUSK, PDGFRα(PDGFRA), PDGFRα(PDGFRA) [D842V], PDGFRα(PDGFRA) [T674I], PDGFRα(PDGFRA) [V561D], PDGFRβ(PDGFRB), RET, RET [G691S], RET [M918T], RET [S891A], RET [Y791F], TNK1, TRKA(NTRK1), TYRO3
BTSU03	Biotin-Modified Erktide	Erk1(MAPK3), Erk2(MAPK1), MINK(MINK1), p38α(MAPK14), p38β(MAPK11), p38γ(MAPK12), p38δ(MAPK13)
BTSU04	Biotin-Blk/Lyntide	BRK(PTK6), EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHB1, EPHB2, EPHB3, EPHB4, FAK(PTK2), PYK2(PTK2B), SRM(SRMS), SYK, TIE2(TEK), ZAP70
BTSU05	Biotin-GS peptide	CaMK1α(CAMK1), CaMK2α(CAMK2A), CaMK2β(CAMK2B), CaMK2γ(CAMK2G), CaMK2δ(CAMK2D), CaMK4, DCAMKL2, MAPKAPK2, MAPKAPK3, MAPKAPK5, MELK, NEK4, PASK, PHKG1, PHKG2, PKD1(PRKD1), PKD2(PRKD2), PKD3(PRKD3), TSSK1, TSSK2, TSSK3
BTSU06	Biotin-DYRKtide	CDK4/CycD3, CDK6/CycD3, CLK1, CLK2, CLK3, DYRK1A, DYRK1B, DYRK2, DYRK3, HIPK1, HIPK2, HIPK3, HIPK4, MSSK1(STK23), SRPK2
BTSU07	Biotin-IRS1	DDR1, DDR2, IGF1R, INSR, IRR(INSRR), MST1(STK4), MST2(STK3), ROS(ROS1)
BTSU08	Biotin-CHKtide	BRSK1, BRSK2, CHK1(CHEK1), CHK2(CHEK2), MARK1, MARK2, MARK3, MARK4, NIM1K(MGC42105), NuaK1, NuaK2
BTSU09	Biotin-Kemptide	AurA(AURKA), AurA(AURKA)/TPX2, AurB(AURKB)/INCENP, AurC(AURKC), CGK2(PRKG2), PGK(PRKG1), PKACα(PRKACA), PKACβ(PRKACB), PKACγ(PRKACG), PRKX
BTSU10	Biotin-PKC peptide	PKCα(PRKCA), PKCβ1(PRKCB1), PKCβ2(PRKCB2), PKCγ(PRKCG), PKCδ(PRKCD), PKCε(PRKCE), PKCζ(PRKCZ), PKCη(PRKCH), PKCθ(PRKCQ), PKCι(PRKCI)
BTSU11	Biotin-CDK7 peptide	NEK1, NEK2, NEK6, NEK7, NEK9
BTSU12	Biotin-CKtide	CK1α(CSNK1A1), CK1γ1(CSNK1G1), CK1γ2(CSNK1G2), CK1γ3(CSNK1G3), CK1δ(CSNK1D), CK1ε(CSNK1E), TBK1
BTSU13	Biotin-Modified Histone H1	CDK1(CDC2)/CycB1, CDK2/CycA2, CDK2/CycE1, CDK3/CycE1, CDK5/p25
BTSU14	Biotin-S6K2 peptide	HPK1(MAP4K1), MAP4K2, MAP4K5(KHS1), p70S6K(RPS6KB1), p70S6Kβ(RPS6KB2), PIM1, PIM2, PIM3
BTSU15	Biotin-SGKtide	LATS1/MOBKL1A, LATS2/MOBKL1A, NDR1(STK38), NDR2(STK38L), PAK4, PAK6, SGK, SGK2, SGK3(SGKL)
BTSU16	Biotin-ABLtide	ABL(ABL1), ABL(ABL1) [E255K], ABL(ABL1) [T315I], ARG(ABL2)
BTSU17	Biotin-DAPK1tide	DAPK1, MRCKα(CDC42BPA), MRCKβ(CDC42BPB), PAK2, PAK5(PAK7)
BTSU18	Biotin-Moesin-derived peptide	HGK(MAP4K4), LOK(STK10), MST3(STK24), MST4, SLK, TNIK
BTSU19	Biotin-Crosstide	AKT1, AKT2, AKT3, MSK1(RPS6KA5), MSK2(RPS6KA4)
BTSU20	Biotin-S6K peptide	RSK1(RPS6KA1), RSK2(RPS6KA3), RSK3(RPS6KA2), RSK4(RPS6KA6)
BTSU21	Biotin-CDC25ctide	PLK1, PLK3
BTSU22	Biotin-CREBtide-p	GSK3α(GSK3A), GSK3β(GSK3B)
BTSU23	Biotin-Histone H3 peptide	CRIK(CIT), Haspin(GSG2), PBK
BTSU24	Biotin-LIMKtide	PAK1, ROCK1, ROCK2
BTSU25	Biotin-CK2tide	CK2α1/β(CSNK2A1/B), CK2α2/β(CSNK2A2/B)
BTSU26	Biotin-SAMS peptide	AMPKα1/β1/γ1(PRKAA1/B1/G1), AMPKα2/β1/γ1(PRKAA2/B1/G1)
BTSU27	Biotin-SPAKtide	WNK1, WNK2, WNK3, WNK4
BTSU28	Biotin-AMARA peptide	QIK(SNF1LK2), SIK(SNF1LK), SIK3(QSK)
BTSU29	Biotin-CDK9 substrate	CDK9/CycT1
BTSU30	Biotin-CTD3 peptide	CDK7/CycH/MAT1
BTSU32	Biotin-JAK1 substrate peptide	JAK1
BTSU33	Biotin-MCM2 peptide
BTSU35	Biotin-PDHKtide	PDHK2(PDK2), PDHK4(PDK4)
BTSU39	Biotin-WASP peptide	ACK(TNK2)
BTSU40	Biotin-EEF2Ktide	EEF2K
BTSU41	Biotin-IRAK1 peptide	IRAK4
BTSU42	Biotin-IκBα peptide	IKKε(IKBKE)
BTSU43	Biotin-MLCtide	skMLCK(MYLK2)
BTSU45	Biotin-Synapsin peptide	CaMK1δ(CAMK1D)
BTSU46	Biotin-TAOKtide	TAOK2
BTSU47	Biotin-H2A peptide	BUB1/BUB3
BTSU48	Biotin-LRRKtide	TAK1-TAB1(MAP3K7)
BTSU70	Biotin-SRPKtide	MNK1(MKNK1), MNK2(MKNK2)