Support

F.A.Q.

Protein Kinases

QPack size of protein kinase products?
A

Starting from 5ug, our kinase products are available in 100, 200, 500, and 1000ug per vial. Some kinases are available in only 5ug. 50ug is minimum for inactive & inactive-mutant kinases. For a bulk amount, please inquire.

QI am interested in protein kinase which is not on the Carna's list.
A

Please inquire us. Some may be under development. Also, we can produce mutated forms of specific protein kinase upon request.

QWhat is the origin of Carna's kinase product?
A

All protein kinases among the list are human recombinant protein.

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Profiling Services

QHow do I appy for the profiling service?
A

After an agreement is concluded, please fill out the application form and submit it to us via email.

QHow do I check on the details of profiling service?
A

Please download the latest kinase profiling book from our website. Detailed information about each kinase, assay condition, and more can be checked on the book.

QHow do I prepare test samples?
A

The required volume of samples is 500uL each if less than 100 target kinases, 1000uL each for over 100 kinases. Please prepare samples in 100% DMSO, 100x of the highest test concentration you selected. Please click here for more details.

QWhen will I receive my profiling result?
A

Normally, 2-3 weeks after the receipt of compounds, you will receive a result in an encrypted file via email.

QHow many kinases are selectable for profiling?
A

342 kinases are profiling available. You can select from just one kinase.

QHow much does it cost for the service?
A

Please inquire us with the number of sample, kinase, and concentration. All assays are performed in duplicate basically.

QIs the panel assay available?
A

Carna is offering four unique pre-selected panels. You can also select additonal kinases to the combination of a panel.

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Assay Kits & Development

QComponent of assay kit?
A

The component varies by a platform. Please click here for more details.

QWhen will I receive the product?
A

Our assay kit is custom-made. Normally, 2-3 weeks for the delivery.

QHow do I refer to the protocol of assay kit in advance of ordering?
A

You can download and check the protocol of each platform on our website.

QCan I order the component separately?
A

After your purchase of a kit, each component of the kit can be ordered separately.

QWhat is the minimum order size of assay kits?
A

100dp per set for ELISA and 400dp for other platforms.

QIs there anything I have to prepare before using assay kits?
A

Additional materials are required for IMAPTM, ELISA, and TR-FRET kits. Please refer to the first page of each protocol.

QCan I order IMAPTM beads?
A

IMAPTM beads can be sold upon request.

QWhat type of plate I have to prepare?
A

Please refer to each protocol for the name of manufacturer and catalogue number of plate which Carna recommends.

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Crystallography

QPack size of crystal-grade protein kinase products?
A

Please feel free to inquire.

QCan I have a detailed sequence of crystal-grade protein kinase in advance of ordering?
A

A sequence is partially disclosable. Please inquire.

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Detection of Protein : Protein Interactions

QWhat should I use as the luciferase substrate?
A

We recommend using Carna’s Split Glow Cell Assay Reagent (PXR-SG001), developed exclusively for our Split Luciferase Assay. Its composition is optimized for this assay to generate the brightest luminescence signal. When detecting a signal inside a living cell (as opposed to a cell lysate), use D-Luciferin (approximately 1.0 mM) dissolved in PBS or HBSS reagent.

QWhat instrument should I use for reading?
A

Use any plate reader applicable to bioluminescence detection, or a tube-type luminometer. A detector with higher sensitivity is better.

QAre Carna’s luciferase assays applicable for imaging?
A

Yes. The emerald luciferase used in our split luciferase system emits a very strong signal, which enables you to image real time signals at a single cell level for several hours. Please see the video of GPCR-arrestin imaging data on our website. Use diluted D-Luciferin in medium (approximately 1.0 mM) for the substrate in a living cell. Please contact us for detailed assay conditions.

QAre ProbeX’s luciferase assays applicable to the detection of PPIs?
A

Yes. These assays have been used to detect signals not only for GPCR studies but also for protein protein interactions inside a cell, such as FKBP-FRB binding. Whether our split luciferase generates a detectable signal when measuring protein protein interactions relies on various factors---protein structural stability, expression level inside a cell, proximity of the two luciferase fragments during the interaction, etc. If you wish to develop a PPI assay by the use of our luciferase technology, please contact us.

QWhat are the advantages of our luciferase technology compared to other similar systems?
A

Emerald luciferase in our split luciferase assay shows higher stability inside a cell and can emit a signal fifteen times brighter than firefly luciferase. Using our Split Glow Cell Assay Reagent (PXR-SG001), which can reduce background emission, you can detect stimuli-responsive PPI signals with high sensitivity. Its ability to detect a signal in cells for GPCR screening in just ten minutes after ligand stimulation also indicates that ProbeX’s luciferase is highly useful for high throughput screening of compounds. Its applicability for imaging in living cells and animals additionally makes it a valuable tool in evaluating compounds in a living cell or animal.

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Others

QHow can I place an order?
A

We have a 100% wholly-owned subsidiary in the US and also distributors in Europe and Asia. Please contact us for more details.

QPayment term?
A

The condition is T/T 30 days.

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Please refer for the other questions from here.

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