カルナバイオサイエンス株式会社

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ルシフェラーゼタンパク質
相互作用 安定発現細胞株

スプリットされたブラジル産ヒカリコメツキムシ由来のルシフェラーゼ(Emerald Luc, E-Luc)が細胞内で会合すると強い蛍光を発します。このスプリットルシフェラーゼシステムを用いれば,GPCRをはじめ細胞内の様々なタンパク質間相互作用を簡便かつ高感度に発光シグナルとして測定することが可能です。

カルナではルシフェラーゼシステム導入済みの安定発現細胞株を取り揃えております。リスト上の安定発現細胞株は、弊社での品質チェックを施した後すぐにお届けが可能です。お届けする細胞株には反応検出用の試薬(Split Glow)が添付されており、追加で試薬単品でのご購入も可能です。また、リストにない細胞株につきましては、お客様のニーズに合わせてご希望の安定発現細胞株の構築も承ります。お気軽にご相談ください。

スプリットルシフェラーゼテクノロジー

動画マークの付いた細胞株はリアルタイムモニタリングの動画がご覧いただけます。

GPCR
Cat.# Target GPCR and Description
PXC-001-1
ADCYAP1R1 Adenylate cyclase activating polypeptide 1 (pituitary) receptor type I Real Time Monitoring
Receptor Family
VIP and PACAP
Coupling
Gs
Accession Number
NM_001118
β-Arrestin Isoform
β- Arrestin-1
Cell Line
HEK293
Species
Human
Cat.# Target GPCR and Description
PXC-002-2
ADRA2A Alpha-2-adrenergic receptor Real Time Monitoring
Receptor Family
Adrenoceptor
Coupling
Gi
Accession Number
NM_000681
β-Arrestin Isoform
β- Arrestin-2
Cell Line
HEK293
Species
Human
Cat.# Target GPCR and Description
PXC-003-1
ADRB2 Beta-2-adrenergic receptor Real Time Monitoring
Receptor Family
Adrenoceptor
Coupling
Gs
Accession Number
NM_000024
β-Arrestin Isoform
β- Arrestin-1
Cell Line
HEK293
Species
Human
Cat.# Target GPCR and Description
PXC-003-2
ADRB2 Beta-2-adrenergic receptor Real Time Monitoring
Receptor Family
Adrenoceptor
Coupling
Gs
Accession Number
NM_000024
β-Arrestin Isoform
β- Arrestin-2
Cell Line
HEK293
Species
Human
Cat.# Target GPCR and Description
PXC-004-1
AGTR1 Angiotensin II receptor, type 1
Receptor Family
Angiotensin
Coupling
Gq
Accession Number
NM_000685
β-Arrestin Isoform
β- Arrestin-1
Cell Line
HEK293
Species
Human
Cat.# Target GPCR and Description
PXC-004-2
AGTR1 Angiotensin II receptor, type 1
Receptor Family
Angiotensin
Coupling
Gq
Accession Number
NM_000685
β-Arrestin Isoform
β- Arrestin-2
Cell Line
HEK293
Species
Human
Cat.# Target GPCR and Description
PXC-005-1
AGTRL1 Apelin receptor
Receptor Family
Apelin
Coupling
Gi
Accession Number
NM_005161
β-Arrestin Isoform
β- Arrestin-1
Cell Line
HEK293
Species
Human
Cat.# Target GPCR and Description
PXC-005-2
AGTRL1 Apelin receptor
Receptor Family
Apelin
Coupling
Gi
Accession Number
NM_005161
β-Arrestin Isoform
β- Arrestin-2
Cell Line
HEK293
Species
Human
Cat.# Target GPCR and Description
PXC-006-1
CCKBR Cholecystokinin B receptor Real Time Monitoring
Receptor Family
Cholecystokinin
Coupling
Gq
Accession Number
NM_176875
β-Arrestin Isoform
β- Arrestin-1
Cell Line
HEK293
Species
Human
Cat.# Target GPCR and Description
PXC-006-2
CCKBR Cholecystokinin B receptor
Receptor Family
Cholecystokinin
Coupling
Gq
Accession Number
NM_176875
β-Arrestin Isoform
β- Arrestin-2
Cell Line
HEK293
Species
Human
Cat.# Target GPCR and Description
PXC-007-1
CCR7 CC chemokine receptor 7 (human)
Receptor Family
Chemokine
Coupling
Gi
Accession Number
NM_001838
β-Arrestin Isoform
β- Arrestin-1
Cell Line
HEK293
Species
Human
Cat.# Target GPCR and Description
PXC-007-2
CCR7 CC chemokine receptor 7 (human)
Receptor Family
Chemokine
Coupling
Gi
Accession Number
NM_001838
β-Arrestin Isoform
β- Arrestin-2
Cell Line
HEK293
Species
Human
Cat.# Target GPCR and Description
PXC-008-1
EDG1 Endothelial differentiation protein/sphingosine-1-phosphate receptor 1
Receptor Family
Lysophospholipid
Coupling
Gi
Accession Number
NM_001400
β-Arrestin Isoform
β- Arrestin-1
Cell Line
HEK293
Species
Human
Cat.# Target GPCR and Description
PXC-008-2
EDG1 Endothelial differentiation protein/sphingosine-1-phosphate receptor 1
Receptor Family
Lysophospholipid
Coupling
Gi
Accession Number
NM_001400
β-Arrestin Isoform
β- Arrestin-2
Cell Line
HEK293
Species
Human
Cat.# Target GPCR and Description
PXC-009-2
ENDRA Endothelin receptor type A
Receptor Family
Endothelin
Coupling
Gq
Accession Number
NM_001957
β-Arrestin Isoform
β- Arrestin-2
Cell Line
HEK293
Species
Human
Cat.# Target GPCR and Description
PXC-010-1
ENDRB Endothelin receptor type B
Receptor Family
Endothelin
Coupling
Gs
Accession Number
NM_000115
β-Arrestin Isoform
β- Arrestin-1
Cell Line
HEK293
Species
Human
Cat.# Target GPCR and Description
PXC-010-2
ENDRB Endothelin receptor type B
Receptor Family
Endothelin
Coupling
Gs
Accession Number
NM_000115
β-Arrestin Isoform
β- Arrestin-2
Cell Line
HEK293
Species
Human
Cat.# Target GPCR and Description
PXC-011-1
EP4 Prostaglandin E2 receptor 4 Real Time Monitoring
Receptor Family
Prostanoid
Coupling
Gs
Accession Number
NM_000958
β-Arrestin Isoform
β- Arrestin-1
Cell Line
HEK293
Species
Human
Cat.# Target GPCR and Description
PXC-011-2
EP4 Prostaglandin E2 receptor 4 Real Time Monitoring
Receptor Family
Prostanoid
Coupling
Gs
Accession Number
NM_000958
β-Arrestin Isoform
β- Arrestin-2
Cell Line
HEK293
Species
Human
Cat.# Target GPCR and Description
PXC-012-2
NPY1R Neuropeptide Y receptor Y1
Receptor Family
Neuropeptide Y
Coupling
Gi
Accession Number
NM_000909
β-Arrestin Isoform
β- Arrestin-2
Cell Line
HEK293
Species
Human
Cat.# Target GPCR and Description
PXC-013-1
OPRM1 Opioid receptor, mu 1 Real Time Monitoring
Receptor Family
Opioid
Coupling
Gi
Accession Number
NM_000914
β-Arrestin Isoform
β- Arrestin-1
Cell Line
HEK293
Species
Human
Cat.# Target GPCR and Description
PXC-013-2
OPRM1 Opioid receptor, mu 1 Real Time Monitoring
Receptor Family
Opioid
Coupling
Gi
Accession Number
NM_000914
β-Arrestin Isoform
β- Arrestin-2
Cell Line
HEK293
Species
Human
Cat.# Target GPCR and Description
PXC-014-1
SSTR2 Somatostatin receptor 2
Receptor Family
Somatostatin
Coupling
Gi
Accession Number
NM_001050
β-Arrestin Isoform
β- Arrestin-1
Cell Line
HEK293
Species
Human
Cat.# Target GPCR and Description
PXC-014-2
SSTR2 Somatostatin receptor 2
Receptor Family
Somatostatin
Coupling
Gi
Accession Number
NM_001050
β-Arrestin Isoform
β- Arrestin-2
Cell Line
HEK293
Species
Human
Cat.# Target GPCR and Description
PXC-015-1
EDG3 Endothelial differentiation protein/sphingosine-1-phosphate receptor 3
Receptor Family
Lysophospholipid
Coupling
Gi
Accession Number
NM_005226.2
β-Arrestin Isoform
β-Arrestin-1
Cell Line
HEK293
Species
Human
タンパク相互作用
Cat.# Target Description
PXC-PPI-1
Accession Number (α)
NM_001530
Accession Number (β)
NM_001668
Cell Line
HEK293
Species
Human
Cat.# Target Description
PXC-PPI-2
Accession Number (α)
NM_020529
Accession Number (β)
NM_001256856
Cell Line
HEK293
Species
Human
Cat.# Target Description
PXC-PPI-3
Accession Number (ARIA)
DQ462572
Accession Number (PTEN)
U96180
Cell Line
HEK293
Species
Human
試薬
Cat.# Name of Product
PXR-SG001 Split Glow Cell Assay Reagent
取扱説明書(英語版のみ)