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HTRF™/LanthaScreen™ Kinase Assay Support
HTRF™ (Revvity) and LanthaScreen™ (ThermoFisher Scientific) kinase assays utilize time-resolved fluorescence resonance energy transfer (TR-FRET) technology to measure the affinity between a kinase and a compound. These assays are performed in a homogeneous reaction format, offering high throughput and making them ideal for drug screening applications.
TR-FRET assays utilizing Carna’s biotinylated kinase proteins do not require antibodies, allowing for highly simplified assay setup and enabling the development of kinase assays across a broad range of targets with excellent reproducibility.
To support your HTRF™ and LanthaScreen™ kinase assay development for compound evaluation and screening, Carna provides assay conditions measured using our kinase proteins.
The data obtained under the assay conditions introduced on this page can be viewed in the PDF attached in the Sample Activity Data column. All the Biotinylated Protein Kinases, and assay buffer products used to obtain these data are available for purchase from Carna.
For product inquiries, questions, or consultations, please contact us from here or at info@carnabio.com.
HTRF™/LanthaScreen™ Kinase Assay Reaction Conditions
| Kinase Name | Protein Kinase | Tracer （Please purchase from each respective supplier/manufacturer） |
Assay Buffer |
Sample Affinity Data |
||||
|---|---|---|---|---|---|---|---|---|
| Tag | Carna Product Name | Cat. No. | Product Name | Cat. No. | Manufacturer | Cat. No. | ||
| ABL(ABL1) | BTN | BTN-ABL(ABL1) | 08-401-20N | Dasatinib-Red | 62KB02REDC | Revvity | ASBF01 | |
| ABL(ABL1)[T315I] | BTN | BTN-ABL(ABL1)[T315I] | 08-493-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| BTN | BTN-ABL(ABL1)[T315I] | 08-493-20N | Dasatinib-Red | 62KB02REDC | Revvity | ASBF01 | ||
| ACTR2B(ACVR2B) | BTN | BTN-ACTR2B(ACVR2B) | 09-432-20N | Kinase Tracer 178 | PV5593 | Thermo Fisher Scientific | ASBF01 | |
| AKT1 | BTN | BTN-AKT1 | 01-401-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| AKT2 | BTN | BTN-AKT2 | 01-402-20N | Kinase Tracer 236 | PV5592 | Thermo Fisher Scientific | ASBF01 | |
| ALK | BTN | BTN-ALK | 08-405-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| ALK[C1156Y] | BTN | BTN-ALK[C1156Y] | 08-730-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| ALK[F1174L] | BTN | BTN-ALK[F1174L] | 08-719-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| ALK[F1174L／G1202R] | BTN | BTN-ALK[F1174L／G1202R] | 08-768-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| ALK[G1202R] | BTN | BTN-ALK[G1202R] | 08-744-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| ALK[G1269A] | BTN | BTN-ALK[G1269A] | 08-737-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| ALK[I1171N] | BTN | BTN-ALK[I1171N] | 08-766-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| ALK[I1171N／L1198F] | BTN | BTN-ALK[I1171N／L1198F] | 08-767-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| ALK[I1171S] | BTN | BTN-ALK[I1171S] | 08-769-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| ALK[L1196M] | BTN | BTN-ALK[L1196M] | 08-429-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| ALK[L1196M／G1202R] | BTN | BTN-ALK[L1196M／G1202R] | 08-756-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| ALK[L1198F] | BTN | BTN-ALK[L1198F] | 08-771-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| ALK[R1275Q] | BTN | BTN-ALK[R1275Q] | 08-720-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| ALK[V1180L] | BTN | BTN-ALK[V1180L] | 08-770-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| AMPKα1/β1/γ1 | BTN | BTN-AMPKα1/β1/γ1 | 02-413-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| AMPKα1/β2/γ1 | BTN | BTN-AMPKα1/β2/γ1 | 02-447-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| AurA | BTN | BTN-AurA | 05-401-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| AurB(AURKB)／INCENP | BTN | BTN-AurB(AURKB)／INCENP | 05-402-21N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| AXL | BTN | BTN-AXL | 08-407-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| BARK1(ADRBK1) | BTN | BTN-BARK1(ADRBK1) | 01-411-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| BMX | BTN | BTN-BMX | 08-479-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| BRAF | BTN | BTN-BRAF | 09-422-20N | Kinase Tracer 178 | PV5593 | Thermo Fisher Scientific | ASBF01 | |
| BRK(PTK6) | BTN | BTN-BRK(PTK6) | 08-465-20N | Dasatinib-Red | 62KB02REDC | Revvity | ASBF01 | |
| BTN | BTN-BRK(PTK6)[non-activated] | 08-465-23N | Dasatinib-Red | 62KB02REDC | Revvity | ASBF01 | ||
| BTK | BTN | BTN-BTK | 08-480-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| BTN | BTN-BTK[non-activated] | 08-480-23N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | ||
| BTK[C481S] | BTN | BTN-BTK[C481S] | 08-417-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| BTN | BTN-BTK[C481S][non-activated] | 08-417-23N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | ||
| BTK[M437R] | BTN | BTN-BTK[M437R] | 08-705-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| BTN | BTN-BTK[M437R][non-activated] | 08-705-23N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | ||
| BTK[T316A] | BTN | BTN-BTK[T316A] | 08-418-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| BTN | BTN-BTK[T316A][non-activated] | 08-418-23N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | ||
| BTK[T474I] | BTN | BTN-BTK[T474I] | 08-419-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| BTN | BTN-BTK[T474I][non-activated] | 08-419-23N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | ||
| BTK[T474S] | BTN | BTN-BTK[T474S] | 08-420-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| BTN | BTN-BTK[T474S][non-activated] | 08-420-23N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | ||
| BTK[V416L] | BTN | BTN-BTK[V416L] | 08-706-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| BTN | BTN-BTK[V416L][non-activated] | 08-706-23N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | ||
| CaMK2β(CAMK2B) | BTN | BTN-CaMK2β(CAMK2B) | 02-410-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| CaMK2δ(CAMK2D) | BTN | BTN-CaMK2δ(CAMK2D) | 02-411-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| CDK10(PISSLRE)／CycQ | BTN | BTN-CDK10(PISSLRE)／CycQ | 04-471-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| CDK19(CDC2L6) | BTN | BTN-CDK19(CDC2L6) | 04-730-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| CDK19(CDC2L6)／CycC | BTN | BTN-CDK19(CDC2L6)／CycC | 04-412-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| CDK2／CycA2 | BTN | BTN-CDK2／CycA2 | 04-403-20N | Kinase Tracer 222 | PV6121 | Thermo Fisher Scientific | ASBF01 | |
| BTN | BTN-CDK2／CycA2 | 04-403-20N | Kinase Tracer 314 | PV6087 | Thermo Fisher Scientific | ASBF01 | ||
| CDK5／p35 | BTN | BTN-CDK5／p35 | 04-492-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| CDK8 | BTN | BTN-CDK8 | 04-496-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| CDK8／CycC | BTN | BTN-CDK8／CycC | 04-409-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| CDK9／CycK | BTN | BTN-CDK9／CycK | 04-411-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| CDK9／CycT1 | BTN | BTN-CDK9／CycT1 | 04-410-20N | Kinase Tracer 236 | PV5592 | Thermo Fisher Scientific | ASBF01 | |
| CDK9／CycT2 | BTN | BTN-CDK9／CycT2 | 04-421-20N | Kinase Tracer 236 | PV5592 | Thermo Fisher Scientific | ASBF01 | |
| CLK4 | BTN | BTN-CLK4 | 04-429-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| DAPK3 | BTN | BTN-DAPK3 | 02-436-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| DDR1 | BTN | BTN-DDR1 | 08-432-20N | Dasatinib-Red | 62KB02REDC | Revvity | ASBF01 | |
| DDR1(SRC treated) | BTN | BTN-DDR1(SRC treated) | 08-732-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| DDR2 | BTN | BTN-DDR2 | 08-414-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| DYRK1A | BTN | BTN-DYRK1A | 04-430-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| EGFR | BTN | BTN-EGFR | 08-415-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| BTN | BTN-EGFR | 08-415-20N | Dasatinib-Red | 62KB02REDC | Revvity | ASBF01 | ||
| EGFR[C797S] | BTN | BTN-EGFR[C797S] | 08-761-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| BTN | BTN-EGFR[C797S] | 08-761-20N | Dasatinib-Red | 62KB02REDC | Revvity | ASBF01 | ||
| EGFR[C797S／L858R] | BTN | BTN-EGFR[C797S／L858R] | 08-763-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| BTN | BTN-EGFR[C797S／L858R] | 08-763-20N | Dasatinib-Red | 62KB02REDC | Revvity | ASBF01 | ||
| EGFR[d746-750] | BTN | BTN-EGFR[d746-750] | 08-427-20N | Dasatinib-Red | 62KB02REDC | Revvity | ASBF01 | |
| EGFR[d746-750／C797S] | BTN | BTN-EGFR[d746-750／C797S] | 08-764-20N | Dasatinib-Red | 62KB02REDC | Revvity | ASBF01 | |
| EGFR[L858R] | BTN | BTN-EGFR[L858R] | 08-402-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| BTN | BTN-EGFR[L858R] | 08-402-20N | Dasatinib-Red | 62KB02REDC | Revvity | ASBF01 | ||
| EGFR[T790M] | BTN | BTN-EGFR[T790M] | 08-494-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| BTN | BTN-EGFR[T790M] | 08-494-20N | Dasatinib-Red | 62KB02REDC | Revvity | ASBF01 | ||
| EGFR[T790M／C797S／L858R] | BTN | BTN-EGFR[T790M／C797S／L858R] | 08-759-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| EGFR[T790M／L858R] | BTN | BTN-EGFR[T790M／L858R] | 08-410-20N | Dasatinib-Red | 62KB02REDC | Revvity | ASBF01 | |
| BTN | BTN-EGFR[T790M／L858R] | 08-410-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | ||
| EPHA2 | BTN | BTN-EPHA2 | 08-421-20N | Dasatinib-Red | 62KB02REDC | Revvity | ASBF01 | |
| BTN | BTN-EPHA2[non-activated] | 08-421-23N | Dasatinib-Red | 62KB02REDC | Revvity | ASBF01 | ||
| FAK(PTK2) | BTN | BTN-FAK(PTK2) | 08-437-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| FGFR1 | BTN | BTN-FGFR1 | 08-435-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| BTN | BTN-FGFR1[non-activated] | 08-435-23N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | ||
| FGFR2 | BTN | BTN-FGFR2 | 08-434-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| BTN | BTN-FGFR2[non-activated] | 08-434-23N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | ||
| FGFR2[C491A／V564L] | BTN | BTN-FGFR2[C491A／V564L] | 08-906-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| BTN | BTN-FGFR2[C491A／V564L][non-activated] | 08-906-23N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | ||
| FGFR2[E565A] | BTN | BTN-FGFR2[E565A] | 08-907-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| BTN | BTN-FGFR2[E565A][non-activated] | 08-907-23N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | ||
| FGFR2[K659M] | BTN | BTN-FGFR2[K659M] | 08-927-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| BTN | BTN-FGFR2[K659M][non-activated] | 08-927-23N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | ||
| FGFR2[L617V] | BTN | BTN-FGFR2[L617V] | 08-926-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| BTN | BTN-FGFR2[L617V][non-activated] | 08-926-23N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | ||
| FGFR2[M537I] | BTN | BTN-FGFR2[M537I] | 08-922-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| BTN | BTN-FGFR2[M537I][non-activated] | 08-922-23N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | ||
| FGFR2[M537I／M538L] | BTN | BTN-FGFR2[M537I／M538L] | 08-924-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| BTN | BTN-FGFR2[M537I／M538L][non-activated] | 08-924-23N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | ||
| FGFR2[M538L] | BTN | BTN-FGFR2[M538L] | 08-923-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| BTN | BTN-FGFR2[M538L][non-activated] | 08-923-23N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | ||
| FGFR2[N549D] | BTN | BTN-FGFR2[N549D] | 08-908-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| BTN | BTN-FGFR2[N549D][non-activated] | 08-908-23N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | ||
| FGFR2[N549H] | BTN | BTN-FGFR2[N549H] | 08-901-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| BTN | BTN-FGFR2[N549H][non-activated] | 08-901-23N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | ||
| FGFR2[N549K] | BTN | BTN-FGFR2[N549K] | 08-909-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| BTN | BTN-FGFR2[N549K][non-activated] | 08-909-23N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | ||
| FGFR2[N549K／V564F] | BTN | BTN-FGFR2[N549K／V564F] | 08-925-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| BTN | BTN-FGFR2[N549K／V564F][non-activated] | 08-925-23N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | ||
| FGFR2[V564F] | BTN | BTN-FGFR2[V564F] | 08-910-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| BTN | BTN-FGFR2[V564F][non-activated] | 08-910-23N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | ||
| FGFR2[V564I] | BTN | BTN-FGFR2[V564I] | 08-902-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| BTN | BTN-FGFR2[V564I][non-activated] | 08-902-23N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | ||
| FGFR2[V564L] | BTN | BTN-FGFR2[V564L] | 08-904-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| BTN | BTN-FGFR2[V564L][non-activated] | 08-904-23N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | ||
| FGFR3 | BTN | BTN-FGFR3 | 08-433-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| FGFR4 | BTN | BTN-FGFR4 | 08-436-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| FLT3 | BTN | BTN-FLT3 | 08-454-21N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| BTN | BTN-FLT3[non-activated] | 08-454-23N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | ||
| FMS(CSF1R) | BTN | BTN-FMS(CSF1R) | 08-455-21N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| BTN | BTN-FMS(CSF1R)[non-activated] | 08-455-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | ||
| FYN [isoform a] | BTN | BTN-FYN [isoform a] | 08-468-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| GSK3β(GSK3B) | BTN | BTN-GSK3β(GSK3B) | 04-441-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| IGF1R | BTN | BTN-IGF1R | 08-441-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| IKKα(CHUK) | BTN | BTN-IKKα(CHUK) | 05-412-20N | Kinase Tracer 236 | PV5592 | Thermo Fisher Scientific | ASBF01 | |
| IKKβ | BTN | BTN-IKKβ | 05-484-20N | Kinase Tracer 236 | PV5592 | Thermo Fisher Scientific | ASBF01 | |
| INSR | BTN | BTN-INSR | 08-442-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| IRAK4 | BTN | BTN-IRAK4 | 09-445-20N | Kinase Tracer 222 | PV6121 | Thermo Fisher Scientific | ASBF01 | |
| ITK | BTN | BTN-ITK | 08-481-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| JAK2 | BTN | BTN-JAK2 | 08-445-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| KDR | BTN | BTN-KDR | 08-491-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| KIT | BTN | BTN-KIT | 08-456-21N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| BTN | BTN-KIT | 08-456-21N | Sunitinib-Red | 62KB03REDC | Revvity | ASBF01 | ||
| BTN | BTN-KIT[non-activated] | 08-456-23N | Sunitinib-Red | 62KB03REDC | Revvity | ASBF01 | ||
| LATS2／MOBKL1A | BTN | BTN-LATS2／MOBKL1A | 01-424-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| LCK | BTN | BTN-LCK | 08-470-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| LIMK2 | BTN | BTN-LIMK2 | 09-406-20N | Dasatinib-Red | 62KB02REDC | Revvity | ASBF01 | |
| LYNa | BTN | BTN-LYNa | 08-471-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| MAP2K1[inactive] | BTN | BTN-MAP2K1[inactive] | 07-441-10-20N | Kinase Tracer 236 | PV5592 | Thermo Fisher Scientific | ASBF01 | |
| MER(MERTK) | BTN | BTN-MER(MERTK) | 08-408-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| MET | BTN | BTN-MET | 08-451-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| MET[D1228H] | BTN | BTN-MET[D1228H] | 08-440-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| MET[Y1230C] | BTN | BTN-MET[Y1230C] | 08-709-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| MLK1(MAP3K9) | BTN | BTN-MLK1(MAP3K9) | 09-415-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| MLK2(MAP3K10) | BTN | BTN-MLK2(MAP3K10) | 09-416-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| MLK3(MAP3K11) | BTN | BTN-MLK3(MAP3K11) | 09-417-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| MST2(STK3) | BTN | BTN-MST2(STK3) | 07-417-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| NEK9 | BTN | BTN-NEK9 | 05-433-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| NuaK2 | BTN | BTN-NuaK2 | 02-427-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| p38α(MAPK14) | BTN | BTN-p38α(MAPK14) | 04-452-20N | Kinase Tracer 199 | PV5830 | Thermo Fisher Scientific | ASBF01 | |
| p38α(MAPK14)[inactive] | BTN | BTN-p38α(MAPK14)[inactive] | 04-452-10-20N | Kinase Tracer 199 | PV5830 | Thermo Fisher Scientific | ASBF01 | |
| PAK4 | BTN | BTN-PAK4 | 07-426-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| PAK6 | BTN | BTN-PAK6 | 07-428-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| PDGFRα(PDGFRA) | BTN | BTN-PDGFRα(PDGFRA)[non-activated] | 08-457-23N | Dasatinib-Red | 62KB02REDC | Revvity | ASBF01 | |
| PDGFRβ(PDGFRB) | BTN | BTN-PDGFRβ(PDGFRB) | 08-458-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| BTN | BTN-PDGFRβ(PDGFRB)[non-activated] | 08-458-23N | Sunitinib-Red | 62KB03REDC | Revvity | ASBF01 | ||
| PIK3CA[E542K]／PIK3R1 | BTN | BTN-PIK3CA[E542K]／PIK3R1 | 11-413-20N | Kinase Tracer 1710 | PV6088 | Thermo Fisher Scientific | ASBF01 | |
| PIK3CA[E545K]／PIK3R1 | BTN | BTN-PIK3CA[E545K]／PIK3R1 | 11-414-20N | Kinase Tracer 1710 | PV6088 | Thermo Fisher Scientific | ASBF01 | |
| PIK3CA[H1047R]／PIK3R1 | BTN | BTN-PIK3CA[H1047R]／PIK3R1 | 11-415-20N | Kinase Tracer 1710 | PV6088 | Thermo Fisher Scientific | ASBF01 | |
| PIK3CA[P539R]／PIK3R1 | BTN | BTN-PIK3CA[P539R]／PIK3R1 | 11-412-20N | Kinase Tracer 1710 | PV6088 | Thermo Fisher Scientific | ASBF01 | |
| PIK3CA[R88Q]／PIK3R1 | BTN | BTN-PIK3CA[R88Q]／PIK3R1 | 11-411-20N | Kinase Tracer 1710 | PV6088 | Thermo Fisher Scientific | ASBF01 | |
| PIK3CA／PIK3R1 | BTN | BTN-PIK3CA／PIK3R1 | 11-401-20N | Kinase Tracer 1710 | PV6088 | Thermo Fisher Scientific | ASBF01 | |
| PIK3CB／PIK3R1 | BTN | BTN-PIK3CB／PIK3R1 | 11-402-20N | Kinase Tracer 314 | PV6087 | Thermo Fisher Scientific | ASBF01 | |
| PIK3CD／PIK3R1 | BTN | BTN-PIK3CD／PIK3R1 | 11-403-20N | Kinase Tracer 1710 | PV6088 | Thermo Fisher Scientific | ASBF01 | |
| BTN | BTN-PIK3CD／PIK3R1 | 11-403-20N | Kinase Tracer 314 | PV6087 | Thermo Fisher Scientific | ASBF01 | ||
| PIK3CG | BTN | BTN-PIK3CG | 11-404-20N | Kinase Tracer 1710 | PV6088 | Thermo Fisher Scientific | ASBF01 | |
| BTN | BTN-PIK3CG | 11-404-20N | Kinase Tracer 314 | PV6087 | Thermo Fisher Scientific | ASBF01 | ||
| PIKFYVE(PIP5K3) | BTN | BTN-PIKFYVE(PIP5K3) | 11-418-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| PKACα(PRKACA) | BTN | BTN-PKACα(PRKACA) | 01-427-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| PKCα(PRKCA) | BTN | BTN-PKCα(PRKCA) | 01-433-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| PKCη | BTN | BTN-PKCη | 01-438-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| PKN1 | BTN | BTN-PKN1 | 01-444-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| PYK2(PTK2B) | BTN | BTN-PYK2(PTK2B) | 08-438-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| RAF1 | BTN | BTN-RAF1 | 09-425-20N | Kinase Tracer 178 | PV5593 | Thermo Fisher Scientific | ASBF01 | |
| RAF1(FL)[Y340E／Y341E] | BTN | BTN-RAF1(FL)[Y340E／Y341E] | 09-427-20N | Kinase Tracer 178 | PV5593 | Thermo Fisher Scientific | ASBF01 | |
| RET | BTN | BTN-RET | 08-459-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| BTN | BTN-RET | 08-459-20N | Kinase Tracer 236 | PV5592 | Thermo Fisher Scientific | ASBF01 | ||
| BTN | BTN-RET[non-activated] | 08-459-23N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | ||
| RET[V804M] | BTN | BTN-RET[V804M] | 08-460-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| BTN | BTN-RET[V804M] | 08-460-20N | Kinase Tracer 236 | PV5592 | Thermo Fisher Scientific | ASBF01 | ||
| BTN | BTN-RET[V804M][non-activated] | 08-460-23N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | ||
| BTN | BTN-RET[V804M][non-activated] | 08-460-23N | Kinase Tracer 236 | PV5592 | Thermo Fisher Scientific | ASBF01 | ||
| SRC | BTN | BTN-SRC | 08-473-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| SYK | BTN | BTN-SYK | 08-476-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| BTN | BTN-SYK[non-activated] | 08-476-23N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | ||
| TAK1-TAB1(MAP3K7) | BTN | BTN-TAK1-TAB1(MAP3K7) | 09-419-21C | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| TBK1 | BTN | BTN-TBK1 | 05-415-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| TEC | BTN | BTN-TEC | 08-482-20N | Dasatinib-Red | 62KB02REDC | Revvity | ASBF01 | |
| BTN | BTN-TEC[non-activated] | 08-482-23N | Dasatinib-Red | 62KB02REDC | Revvity | ASBF01 | ||
| TGFβR1(TGFBR1) | BTN | BTN-TGFβR1(TGFBR1) | 09-441-20N | Kinase Tracer 178 | PV5593 | Thermo Fisher Scientific | ASBF01 | |
| TIE2(TEK) | BTN | BTN-TIE2(TEK) | 08-485-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| TNIK | BTN | BTN-TNIK | 07-438-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| TRKA(NTRK1) | BTN | BTN-TRKA(NTRK1) | 08-486-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| BTN | BTN-TRKA(NTRK1)[non-activated] | 08-486-23N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | ||
| TRKB(NTRK2) | BTN | BTN-TRKB(NTRK2) | 08-487-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| BTN | BTN-TRKB(NTRK2)[non-activated] | 08-487-23N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | ||
| TRKC(NTRK3) | BTN | BTN-TRKC(NTRK3) | 08-497-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| TXK | BTN | BTN-TXK | 08-483-20N | Dasatinib-Red | 62KB02REDC | Revvity | ASBF01 | |
| TYRO3 | BTN | BTN-TYRO3 | 08-409-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
| BTN | BTN-TYRO3[non-activated] | 08-409-23N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | ||
| WEE1 | BTN | BTN-WEE1 | 05-477-20N | Dasatinib-Red | 62KB02REDC | Revvity | ASBF01 | |
| WNK4 | BTN | BTN-WNK4 | 05-482-20N | Kinase Tracer 1710 | PV6088 | Thermo Fisher Scientific | ASBF01 | |
| YES(YES1) | BTN | BTN-YES(YES1) | 08-475-20N | Staurosporine-Red | 62KB01REDC | Revvity | ASBF01 | |
Our HTRF™/LanthaScreen™ Kinase Assay Protocol
- Prepare the assay buffer (20 mM HEPES, 0.01% Triton X-100, 2 mM DTT, pH 7.5).
- Using the assay buffer, prepare 4x Eu-Streptavidin solution, 4x tracer solution, and 4x kinase/metal solution containing additives required for each kinase reaction.
- In each well of a black polystyrene 384-well microplate (Corning #3573), mix 5 µL of assay buffer, 5 µL of 4x kinase/metal solution, 5 µL of 4x tracer solution, and 5 µL of 4x Eu-Streptavidin solution. Incubate at room temperature for 1 hour.
- Measure each well using TR-FRET (Excitation: 320 nm, Emission: 665 nm) with EnVision 2104 (Revvity), and evaluate kinase activity based on the emission ratio (665/615).
