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PhosphoSens™ Kinase Assay Support
Kinase assays utilizing PhosphoSens™ technology (AssayQuant Technologies) enable the acquisition of dynamic kinetic data through real-time and time-course monitoring of enzymatic reactions. The peptide substrate incorporates Sulfonamido-Oxine (Sox), a fluorophore that emits fluorescence upon chelation with Mg2+. When phosphorylated by a kinase, the Sox-containing peptide exhibits increased fluorescence, which can be detected over time. This time-resolved fluorescence signal enhances understanding of reaction mechanisms and supports detailed evaluation of enzyme characteristics and inhibitor effects. With a simple protocol and high throughput capability, this platform is also well-suited for drug screening applications.
To support compound evaluation and screening using the PhosphoSens™ kinase assay in your laboratories, Carna provides optimized assay conditions developed using our protein products for reference.
All assays listed on this page have been optimized using our kinase products. For detailed conditions of each assay, please refer to the attached PDF Sample Activity Data. All the kinase proteins listed are available for purchase from Carna.
For product inquiries, questions, or consultations, please contact us from here or at info@carnabio.com.
PhosphoSens™ Kinase Assay Reaction Conditions
| Kinase Name | Protein Kinase | PhosphoSens Substrate | Sample Activity Data |
||||
|---|---|---|---|---|---|---|---|
| Tag | Carna Product Name | Cat. No. | AssayQuant Cat. No. | Conc.(uM) | Manufacturer | ||
| ABL(ABL1) | HIS | ABL(ABL1) | 08-001 | AQT0032 | 10 µM | AssayQuant Technologies | |
| ABL(ABL1)[E255K] | HIS | ABL(ABL1)[E255K] | 08-094 | AQT0032 | 15 µM | AssayQuant Technologies | |
| ABL(ABL1)[T315I] | HIS | ABL(ABL1)[T315I] | 08-093 | AQT0032 | 15 µM | AssayQuant Technologies | |
| AKT1 | GST | AKT1 | 01-101 | AQT0536 | 15 µM | AssayQuant Technologies | |
| AKT2 | GST | AKT2 | 01-102 | AQT0536 | 15 µM | AssayQuant Technologies | |
| AKT3 | GST | AKT3 | 01-103 | AQT0536 | 15 µM | AssayQuant Technologies | |
| ALK | GST | ALK | 08-518 | AQT0794 | 15 µM | AssayQuant Technologies | |
| ALK[D1203N] | GST | ALK[D1203N] | 08-583 | AQT0794 | 15 µM | AssayQuant Technologies | |
| ALK[G1202R] | GST | ALK[G1202R] | 08-544 | AQT0101 | 15 µM | AssayQuant Technologies | |
| ALK[I1171N] | GST | ALK[I1171N] | 08-566 | AQT0794 | 15 µM | AssayQuant Technologies | |
| ALK[I1171S] | GST | ALK[I1171S] | 08-569 | AQT0794 | 15 µM | AssayQuant Technologies | |
| ALK[I1171T] | GST | ALK[I1171T] | 08-590 | AQT0794 | 15 µM | AssayQuant Technologies | |
| ALK[L1196M／G1202R] | GST | ALK[L1196M／G1202R] | 08-556 | AQT0101 | 10 µM | AssayQuant Technologies | |
| ALK[L1198F] | GST | ALK[L1198F] | 08-571 | AQT0794 | 15 µM | AssayQuant Technologies | |
| ALK[V1180L] | GST | ALK[V1180L] | 08-570 | AQT0794 | 15 µM | AssayQuant Technologies | |
| ARG(ABL2) | GST | ARG(ABL2) | 08-102 | AQT0032 | 10 µM | AssayQuant Technologies | |
| AurA | GST | AurA (AURKA) | 05-101 | AQT0186 | 15 µM | AssayQuant Technologies | |
| AurB(AURKB)／INCENP | GST | AurB(AURKB)／INCENP | 05-102 | AQT0186 | 15 µM | AssayQuant Technologies | |
| AurC | GST | AurC (AURKC) | 05-103 | AQT0186 | 15 µM | AssayQuant Technologies | |
| AXL | GST | AXL | 08-107 | AQT0794 | 15 µM | AssayQuant Technologies | |
| BLK | GST | BLK | 08-164 | AQT0794 | 15 µM | AssayQuant Technologies | |
| BMX | GST | BMX | 08-179 | AQT0794 | 15 µM | AssayQuant Technologies | |
| BRK(PTK6) | GST | BRK(PTK6) | 08-165 | AQT0794 | 15 µM | AssayQuant Technologies | |
| BRSK1 | GST | BRSK1 | 02-115 | AQT0900 | 15 µM | AssayQuant Technologies | |
| BRSK2 | GST | BRSK2 | 02-116 | AQT0900 | 15 µM | AssayQuant Technologies | |
| BTK | GST | BTK | 08-180 | AQT0101 | 15 µM | AssayQuant Technologies | |
| BUB1／BUB3 | GST | BUB1／BUB3 | 05-187 | AQT0345 | 15 µM | AssayQuant Technologies | |
| BUBR1 | GST | BUBR1 (BUB1B) | 05-105 | AQT0683 | 15 µM | AssayQuant Technologies | |
| CaMK2α(CAMK2A) | GST | CaMK2α(CAMK2A) | 02-109 | AQT0356 | 15 µM | AssayQuant Technologies | |
| CAMKK2 | GST | CAMKK2 | 05-108 | AQT1116 | 15 µM | AssayQuant Technologies | |
| CDK1(CDC2)／CycA2 | GST | CDK1(CDC2)／CycA2 | 04-119 | AQT0297 | 15 µM | AssayQuant Technologies | |
| CDK1(CDC2)／CycB1 | GST | CDK1(CDC2)／CycB1 | 04-102 | AQT0297 | 15 µM | AssayQuant Technologies | |
| CDK12(CRKRS)(720-1490aa)／CycK | BTN | BTN-CDK12(CRKRS)(720-1490aa)／CycK | 04-413-20N | AQT0991 | 15 µM | AssayQuant Technologies | |
| CDK2／CycA2 | GST | CDK2／CycA2 | 04-103 | AQT0297 | 15 µM | AssayQuant Technologies | |
| CDK2／CycE1 | GST | CDK2／CycE1 | 04-165 | AQT0297 | 15 µM | AssayQuant Technologies | |
| CDK3／CycE1 | GST | CDK3／CycE1 | 04-104 | AQT0297 | 15 µM | AssayQuant Technologies | |
| CDK4／CycD3 | GST | CDK4／CycD3 | 04-105 | AQT0258 | 15 µM | AssayQuant Technologies | |
| CDK6／CycD1 | GST | CDK6／CycD1 | 04-114 | AQT0258 | 15 µM | AssayQuant Technologies | |
| CDK6／CycD3 | GST | CDK6／CycD3 | 04-107 | AQT0258 | 15 µM | AssayQuant Technologies | |
| CDK7／CycH／MAT1 | GST | CDK7／CycH／MAT1 | 04-108 | AQT0733 | 15 µM | AssayQuant Technologies | |
| CDK9／CycK | GST | CDK9／CycK | 04-111 | AQT1007 | 15 µM | AssayQuant Technologies | |
| CDK9／CycT1 | GST | CDK9／CycT1 | 04-110 | AQT0733 | 15 µM | AssayQuant Technologies | |
| CDK9／CycT2 | GST | CDK9／CycT2 | 04-121 | AQT0733 | 15 µM | AssayQuant Technologies | |
| CHK1(CHEK1) | GST | CHK1(CHEK1) | 02-117 | AQT0694 | 15 µM | AssayQuant Technologies | |
| CHK2(CHEK2) | GST | CHK2(CHEK2) | 02-162 | AQT0694 | 15 µM | AssayQuant Technologies | |
| CK1α | GST | CK1α (CSNK1A1) | 03-101 | AQT0709 | 15 µM | AssayQuant Technologies | |
| CK1γ1 | GST | CK1γ1 (CSNK1G1) | 03-105 | AQT0709 | 15 µM | AssayQuant Technologies | |
| CK1γ2 | GST | CK1γ2 (CSNK1G2) | 03-106 | AQT0709 | 15 µM | AssayQuant Technologies | |
| CK1γ3 | GST | CK1γ3 (CSNK1G3) | 03-107 | AQT0709 | 15 µM | AssayQuant Technologies | |
| CK1δ | GST | CK1δ (CSNK1D) | 03-103 | AQT0709 | 15 µM | AssayQuant Technologies | |
| CK1ε | GST | CK1ε (CSNK1E) | 03-104 | AQT0709 | 15 µM | AssayQuant Technologies | |
| COT | Tag-free | COT (MAP3K8) | 07-301 | AQT0918 | 15 µM | AssayQuant Technologies | |
| CRIK | GST | CRIK (CIT) | 01-104 | AQT0537 | 15 µM | AssayQuant Technologies | |
| CSK | GST | CSK | 08-111 | AQT0794 | 15 µM | AssayQuant Technologies | |
| DDR1(SRC treated) | GST | DDR1(SRC treated) | 08-132 | AQT0280 | 15 µM | AssayQuant Technologies | |
| DDR2 | GST | DDR2 | 08-114 | AQT0235 | 15 µM | AssayQuant Technologies | |
| DYRK1A | GST | DYRK1A | 04-130 | AQT0260 | 20 µM | AssayQuant Technologies | |
| DYRK1B | GST | DYRK1B | 04-131 | AQT0260 | 20 µM | AssayQuant Technologies | |
| EPHA1 | GST | EPHA1 | 08-119 | AQT0658 | 15 µM | AssayQuant Technologies | |
| EPHA2 | GST | EPHA2 | 08-121 | AQT0661 | 15 µM | AssayQuant Technologies | |
| EPHA3 | GST | EPHA3 | 08-122 | AQT0661 | 15 µM | AssayQuant Technologies | |
| EPHA4 | GST | EPHA4 | 08-123 | AQT0661 | 15 µM | AssayQuant Technologies | |
| EPHA5 | GST | EPHA5 | 08-124 | AQT0658 | 15 µM | AssayQuant Technologies | |
| EPHA6 | GST | EPHA6 | 08-125 | AQT0653 | 15 µM | AssayQuant Technologies | |
| EPHA7 | GST | EPHA7 | 08-126 | AQT0653 | 15 µM | AssayQuant Technologies | |
| EPHA8 | GST | EPHA8 | 08-127 | AQT0661 | 15 µM | AssayQuant Technologies | |
| EPHB1 | GST | EPHB1 | 08-128 | AQT0661 | 15 µM | AssayQuant Technologies | |
| EPHB2 | GST | EPHB2 | 08-129 | AQT0661 | 15 µM | AssayQuant Technologies | |
| EPHB3 | GST | EPHB3 | 08-130 | AQT0661 | 15 µM | AssayQuant Technologies | |
| EPHB4 | GST | EPHB4 | 08-131 | AQT0661 | 15 µM | AssayQuant Technologies | |
| Erk1(MAPK3) | GST | Erk1(MAPK3) | 04-142 | AQT0490 | 10 µM | AssayQuant Technologies | |
| Erk2(MAPK1) | GST | Erk2(MAPK1) | 04-143 | AQT0490 | 10 µM | AssayQuant Technologies | |
| FER | GST | FER | 08-139 | AQT0794 | 15 µM | AssayQuant Technologies | |
| FES | GST | FES | 08-140 | AQT0794 | 15 µM | AssayQuant Technologies | |
| FGFR1 | GST | FGFR1 | 08-133 | AQT0794 | 15 µM | AssayQuant Technologies | |
| FGFR2 | GST | FGFR2 | 08-134 | AQT0794 | 15 µM | AssayQuant Technologies | |
| FGFR3 | GST | FGFR3 | 08-135 | AQT0794 | 15 µM | AssayQuant Technologies | |
| FGFR3[V555M] | GST | FGFR3[V555M] | 08-543 | AQT0515 | 15 µM | AssayQuant Technologies | |
| FGFR4 | GST | FGFR4 | 08-136 | AQT0794 | 15 µM | AssayQuant Technologies | |
| FGR | GST | FGR | 08-166 | AQT0661 | 15 µM | AssayQuant Technologies | |
| FLT1 | GST | FLT1 | 08-189 | AQT0794 | 15 µM | AssayQuant Technologies | |
| FLT3 | GST | FLT3 | 08-154 | AQT0794 | 15 µM | AssayQuant Technologies | |
| FLT4 | GST | FLT4 | 08-190 | AQT0794 | 15 µM | AssayQuant Technologies | |
| FMS(CSF1R) | GST | FMS(CSF1R) | 08-155 | AQT0794 | 15 µM | AssayQuant Technologies | |
| FRK | GST | FRK | 08-167 | AQT0794 | 15 µM | AssayQuant Technologies | |
| FYN [isoform a] | GST | FYN [isoform a] | 08-168 | AQT0794 | 15 µM | AssayQuant Technologies | |
| FYN [isoform b] | GST | FYN [isoform b] | 08-531 | AQT0794 | 15 µM | AssayQuant Technologies | |
| HCK | GST | HCK | 08-169 | AQT0794 | 15 µM | AssayQuant Technologies | |
| HER4 | GST | HER4 (ERBB4) | 08-118 | AQT0794 | 15 µM | AssayQuant Technologies | |
| HGK | GST | HGK (MAP4K4) | 07-137 | AQT0135 | 15 µM | AssayQuant Technologies | |
| IGF1R | GST | IGF1R | 08-141 | AQT0794 | 15 µM | AssayQuant Technologies | |
| IKKα(CHUK) | GST | IKKα(CHUK) | 05-112 | AQT0683 | 15 µM | AssayQuant Technologies | |
| INSR | GST | INSR | 08-142 | AQT0794 | 15 µM | AssayQuant Technologies | |
| IRR | GST | IRR (INSRR) | 08-143 | AQT0661 | 15 µM | AssayQuant Technologies | |
| ITK | GST | ITK | 08-181 | AQT0101 | 15 µM | AssayQuant Technologies | |
| JAK2 | HIS | JAK2 | 08-045 | AQT0661 | 15 µM | AssayQuant Technologies | |
| JAK2(JH1 JH2) | GST | JAK2(JH1 JH2) | 08-514 | AQT0661 | 15 µM | AssayQuant Technologies | |
| JAK3 | HIS | JAK3 | 08-046 | AQT0661 | 15 µM | AssayQuant Technologies | |
| KDR | GST | KDR | 08-191 | AQT0794 | 15 µM | AssayQuant Technologies | |
| KIT | GST | KIT | 08-156 | AQT0661 | 15 µM | AssayQuant Technologies | |
| KIT[D816V] | GST | KIT[D816V] | 08-505 | AQT0101 | 15 µM | AssayQuant Technologies | |
| LATS1／MOBKL1A | GST | LATS1／MOBKL1A | 01-523 | AQT0476 | 15 µM | AssayQuant Technologies | |
| LATS2／MOBKL1A | GST | LATS2／MOBKL1A | 01-524 | AQT0476 | 15 µM | AssayQuant Technologies | |
| LCK | GST | LCK | 08-170 | AQT0794 | 15 µM | AssayQuant Technologies | |
| LTK | GST | LTK | 08-106 | AQT0794 | 15 µM | AssayQuant Technologies | |
| LYNa | GST | LYNa | 08-171 | AQT0794 | 15 µM | AssayQuant Technologies | |
| LYNb | GST | LYNb | 08-172 | AQT0794 | 15 µM | AssayQuant Technologies | |
| MAP3K1 | GST | MAP3K1 | 07-103 | AQT0623 | 15 µM | AssayQuant Technologies | |
| MAP3K14 | GST | MAP3K14 | 07-102 | AQT0912 | 15 µM | AssayQuant Technologies | |
| MAPKAPK2 | GST | MAPKAPK2 | 02-142 | AQT0426 | 15 µM | AssayQuant Technologies | |
| MAPKAPK3 | GST | MAPKAPK3 | 02-143 | AQT0425 | 15 µM | AssayQuant Technologies | |
| MAPKAPK5 | GST | MAPKAPK5 | 02-144 | AQT0426 | 15 µM | AssayQuant Technologies | |
| MAPKAPK5 | GST | MAPKAPK5 | 02-144 | AQT1102 | 15 µM | AssayQuant Technologies | |
| MER(MERTK) | GST | MER(MERTK) | 08-108 | AQT0794 | 15 µM | AssayQuant Technologies | |
| MET | GST | MET | 08-151 | AQT0658 | 15 µM | AssayQuant Technologies | |
| MET[D1228N] | GST | MET[D1228N] | 08-120 | AQT0702 | 15 µM | AssayQuant Technologies | |
| MET[Y1230H] | GST | MET[Y1230H] | 08-178 | AQT0658 | 15 µM | AssayQuant Technologies | |
| MLK2(MAP3K10) | GST | MLK2(MAP3K10) | 09-116 | AQT0646 | 15 µM | AssayQuant Technologies | |
| MLK3(MAP3K11) | GST | MLK3(MAP3K11) | 09-117 | AQT0646 | 15 µM | AssayQuant Technologies | |
| MNK1(MKNK1) | GST | MNK1(MKNK1) | 02-145 | AQT0414 | 15 µM | AssayQuant Technologies | |
| MNK2 | GST | MNK2 (MKNK2) | 02-146 | AQT0701 | 15 µM | AssayQuant Technologies | |
| MRCKβ | GST | MRCKβ (CDC42BPB) | 01-108 | AQT0837 | 15 µM | AssayQuant Technologies | |
| MSK1 | GST | MSK1 (RPS6KA5) | 01-147 | AQT0797 | 10 µM | AssayQuant Technologies | |
| MSK2 | GST | MSK2 (RPS6KA4) | 01-148 | AQT0988 | 15 µM | AssayQuant Technologies | |
| MTOR(FRAP)／MLST8 | DYKDDDDK | MTOR(FRAP)／MLST8 | 11-431 | AQT0732 | 20 µM | AssayQuant Technologies | |
| MUSK | GST | MUSK | 08-153 | AQT0031 | 15 µM | AssayQuant Technologies | |
| NEK3 | GST | NEK3 | 05-127 | AQT0685 | 15 µM | AssayQuant Technologies | |
| NEK6 | GST | NEK6 | 05-130 | AQT0688 | 15 µM | AssayQuant Technologies | |
| NEK7 | GST | NEK7 | 05-131 | AQT0887 | 15 µM | AssayQuant Technologies | |
| p38α(MAPK14) | GST | p38α(MAPK14) | 04-152 | AQT0490 | 10 µM | AssayQuant Technologies | |
| p38β | GST | p38β (MAPK11) | 04-153 | AQT0490 | 10 µM | AssayQuant Technologies | |
| p38γ | GST | p38γ (MAPK12) | 04-155 | AQT0652 | 10 µM | AssayQuant Technologies | |
| p38δ | GST | p38δ (MAPK13) | 04-154 | AQT0652 | 10 µM | AssayQuant Technologies | |
| PASK | GST | PASK | 02-128 | AQT0694 | 15 µM | AssayQuant Technologies | |
| PCTAIRE1(CDK16)／CycY | GST | PCTAIRE1(CDK16)／CycY | 04-116 | AQT0258 | 15 µM | AssayQuant Technologies | |
| PDGFRα(PDGFRA) | GST | PDGFRα(PDGFRA) | 08-157 | AQT0661 | 15 µM | AssayQuant Technologies | |
| PDGFRα(PDGFRA)[V561D] | GST | PDGFRα(PDGFRA)[V561D] | 08-507 | AQT0734 | 15 µM | AssayQuant Technologies | |
| PDGFRβ(PDGFRB) | GST | PDGFRβ(PDGFRB) | 08-158 | AQT0661 | 15 µM | AssayQuant Technologies | |
| PIM1 | HIS | PIM1 | 02-054 | AQT0474 | 15 µM | AssayQuant Technologies | |
| PIM2 | GST | PIM2 | 02-155 | AQT0474 | 15 µM | AssayQuant Technologies | |
| PIM3 | GST | PIM3 | 02-156 | AQT0474 | 15 µM | AssayQuant Technologies | |
| PKACα(PRKACA) | GST | PKACα(PRKACA) | 01-127 | AQT0458 | 15 µM | AssayQuant Technologies | |
| PKACβ(PRKACB) | GST | PKACβ(PRKACB) | 01-128 | AQT0458 | 15 µM | AssayQuant Technologies | |
| PKD1 | GST | PKD1 (PRKD1) | 02-157 | AQT0803 | 15 µM | AssayQuant Technologies | |
| PKD2 | GST | PKD2 (PRKD2) | 02-158 | AQT0803 | 15 µM | AssayQuant Technologies | |
| PKD3 | GST | PKD3 (PRKD3) | 02-159 | AQT0803 | 15 µM | AssayQuant Technologies | |
| PRKX | GST | PRKX | 01-130 | AQT0186 | 15 µM | AssayQuant Technologies | |
| PYK2(PTK2B) | GST | PYK2(PTK2B) | 08-138 | AQT0661 | 15 µM | AssayQuant Technologies | |
| RET | GST | RET | 08-159 | AQT0661 | 15 µM | AssayQuant Technologies | |
| RET[G810C／M918T] | GST | RET[G810C／M918T] | 08-580 | AQT0101 | 15 µM | AssayQuant Technologies | |
| RET[V804M] | BTN | BTN-RET[V804M] * | 08-460-20N | AQT0101 | 15 µM | AssayQuant Technologies | |
| ROCK1 | GST | ROCK1 | 01-109 | AQT0839 | 15 µM | AssayQuant Technologies | |
| ROCK2 | GST | ROCK2 | 01-110 | AQT0839 | 15 µM | AssayQuant Technologies | |
| RON | GST | RON (MST1R) | 08-152 | AQT0794 | 15 µM | AssayQuant Technologies | |
| ROS(ROS1) | GST | ROS(ROS1) | 08-163 | AQT0661 | 15 µM | AssayQuant Technologies | |
| ROS(ROS1)[G2032R／L2086F] | GST | ROS(ROS1)[G2032R／L2086F] | 08-587 | AQT0101 | 15 µM | AssayQuant Technologies | |
| RSK1(RPS6KA1) | GST | RSK1(RPS6KA1) | 01-149 | AQT0232 | 15 µM | AssayQuant Technologies | |
| RSK2(RPS6KA3) | GST | RSK2(RPS6KA3) | 01-150 | AQT0232 | 15 µM | AssayQuant Technologies | |
| RSK3(RPS6KA2) | GST | RSK3(RPS6KA2) | 01-151 | AQT0232 | 15 µM | AssayQuant Technologies | |
| RSK4(RPS6KA6) | GST | RSK4(RPS6KA6) | 01-152 | AQT0232 | 15 µM | AssayQuant Technologies | |
| SGK | GST | SGK | 01-158 | AQT0536 | 15 µM | AssayQuant Technologies | |
| SGK2 | GST | SGK2 | 01-159 | AQT0536 | 15 µM | AssayQuant Technologies | |
| SGK3 | GST | SGK3 (SGKL) | 01-160 | AQT0536 | 15 µM | AssayQuant Technologies | |
| SRC | GST | SRC | 08-173 | AQT0794 | 15 µM | AssayQuant Technologies | |
| SRM | GST | SRM (SRMS) | 08-174 | AQT0794 | 15 µM | AssayQuant Technologies | |
| SYK | GST | SYK | 08-176 | AQT0794 | 15 µM | AssayQuant Technologies | |
| TAK1-TAB1(MAP3K7) | BTN | BTN-TAK1-TAB1(MAP3K7) * | 09-419-21C | AQT0356 | 15 µM | AssayQuant Technologies | |
| TAOK2 | GST | TAOK2 | 07-133 | AQT0253 | 15 µM | AssayQuant Technologies | |
| TBK1 | GST | TBK1 | 05-115 | AQT0897 | 15 µM | AssayQuant Technologies | |
| TEC | GST | TEC | 08-182 | AQT0794 | 15 µM | AssayQuant Technologies | |
| TIE2(TEK) | GST | TIE2(TEK) | 08-185 | AQT0794 | 15 µM | AssayQuant Technologies | |
| TLK1 | GST | TLK1 | 05-166 | AQT0686 | 15 µM | AssayQuant Technologies | |
| TLK2 | GST | TLK2 | 05-167 | AQT0686 | 15 µM | AssayQuant Technologies | |
| TNIK | GST | TNIK | 07-138 | AQT0135 | 15 µM | AssayQuant Technologies | |
| TRKA(NTRK1) | GST | TRKA(NTRK1) | 08-186 | AQT0794 | 15 µM | AssayQuant Technologies | |
| TRKB(NTRK2) | GST | TRKB(NTRK2) | 08-187 | AQT0794 | 15 µM | AssayQuant Technologies | |
| TRKC(NTRK3) | GST | TRKC(NTRK3) | 08-197 | AQT0794 | 15 µM | AssayQuant Technologies | |
| TTBK2 | GST | TTBK2 | 03-109 | AQT0686 | 15 µM | AssayQuant Technologies | |
| TTK | GST | TTK | 05-169 | AQT0897 | 15 µM | AssayQuant Technologies | |
| TXK | GST | TXK | 08-183 | AQT0794 | 15 µM | AssayQuant Technologies | |
| TYK2 | GST | TYK2 | 08-147 | AQT0663 | 20 µM | AssayQuant Technologies | |
| TYRO3 | GST | TYRO3 | 08-109 | AQT0794 | 15 µM | AssayQuant Technologies | |
| ULK3 | GST | ULK3 | 05-173 | AQT0686 | 15 µM | AssayQuant Technologies | |
| WEE1 | BTN | BTN-WEE1 | 05-477-20N | AQT0728 | 40 µM | AssayQuant Technologies | |
| YES(YES1) | GST | YES(YES1) | 08-175 | AQT0794 | 15 µM | AssayQuant Technologies | |
| ZAK | GST | ZAK | 09-120 | AQT0753 | 15 µM | AssayQuant Technologies | |
| ZAP70 | GST | ZAP70 | 08-177 | AQT0794 | 15 µM | AssayQuant Technologies | |
PhosphoSens™ Assay Principle & Assay Format
PhosphoSens™ utilizes an improved CSox sensor, in which Sox is introduced into a cysteine residue. When the CSox-conjugated substrate is phosphorylated in the presence of kinase, ATP, and Mg2+, a complex is formed through chelation of Mg2+ with the phosphate group and CSox. Upon excitation, this complex emits fluorescence. .By detecting changes in fluorescence intensity over time, the phosphorylation state of the substrate can be monitored in real time.
（This technology was developed at the Massachusetts Institute of Technology (MIT), and AssayQuant holds the exclusive rights to practice the patent owned by MIT）
（This technology was developed at the Massachusetts Institute of Technology (MIT), and AssayQuant holds the exclusive rights to practice the patent owned by MIT）
- PhosphoSens‑Kinetic（Continuous, Kinetic Fluorescent Intensity (FI) Assays）
- Real-time kinetic analysis can be conducted using purified kinase enzymes or various biological samples (such as cell lysates or tissue homogenates), enabling IC50 determination, mechanism of action (MOA/MOI) studies, and structure-activity relationship (SAR) research.
- PhosphoSens‑Red（Endpoint Time-Resolved Fluorescence (TRF) Assays）
- This TRF (time-resolved fluorescence)-based format is ideal for high-throughput screening (HTS), enabling measurements that fully leverage PhosphoSens™ technology while minimizing compound autofluorescence.
