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PhosphoSens™ キナーゼアッセイサポート
PhosphoSens™（AssayQuant Technologies社）技術を用いたキナーゼアッセイでは、酵素反応をリアルタイムかつ経時的に測定することで動的なキネティックデータの取得が可能です。Mg2+をキレーティングすることで蛍光を発するSulfonamido-Oxine(Sox) を組み込んだペプチド基質はキナーゼによるリン酸化で蛍光を発します。このリン酸化による発光を蛍光強度の増加として経時的に検出したデータは反応メカニズムの理解を深め酵素特性の評価および阻害剤効果の詳細解析に役立ちます。シンプルなプロトコールでスループットが高く薬剤スクリーニングにも最適なプラットフォームです。
お客様のラボでPhosphoSens™キナーゼアッセイを用いて薬剤評価やスクリーニングを行う際のご参考用に、カルナでは自社タンパク質製品を用いて最適化したPhosphoSens™キナーゼアッセイ実施条件を公開しております。
本ページリストにあるアッセイは全てカルナのキナーゼ製品を用いて最適化されています。各アッセイの詳細条件は添付されているPDFデータ内の記載をご覧ください。リスト上のキナーゼタンパク質製品は全て当社で購入可能です。
お問い合わせ、ご質問、ご相談等はこちらよりまたはinfo@carnabio.com からご連絡ください。
PhosphoSens™キナーゼアッセイ 反応条件一覧表
| Kinase Name | Protein Kinase | PhosphoSens Substrate | Sample Activity Data |
||||
|---|---|---|---|---|---|---|---|
| Tag | Carna Product Name | Cat. No. | AssayQuant Cat. No. | Conc.(uM) | Manufacturer | ||
| ABL(ABL1) | HIS | ABL(ABL1) | 08-001 | AQT0032 | 10 µM | AssayQuant Technologies | |
| ABL(ABL1)[E255K] | HIS | ABL(ABL1)[E255K] | 08-094 | AQT0032 | 15 µM | AssayQuant Technologies | |
| ABL(ABL1)[T315I] | HIS | ABL(ABL1)[T315I] | 08-093 | AQT0032 | 15 µM | AssayQuant Technologies | |
| AKT1 | GST | AKT1 | 01-101 | AQT0536 | 15 µM | AssayQuant Technologies | |
| AKT2 | GST | AKT2 | 01-102 | AQT0536 | 15 µM | AssayQuant Technologies | |
| AKT3 | GST | AKT3 | 01-103 | AQT0536 | 15 µM | AssayQuant Technologies | |
| ALK | GST | ALK | 08-518 | AQT0794 | 15 µM | AssayQuant Technologies | |
| ALK[D1203N] | GST | ALK[D1203N] | 08-583 | AQT0794 | 15 µM | AssayQuant Technologies | |
| ALK[G1202R] | GST | ALK[G1202R] | 08-544 | AQT0101 | 15 µM | AssayQuant Technologies | |
| ALK[I1171N] | GST | ALK[I1171N] | 08-566 | AQT0794 | 15 µM | AssayQuant Technologies | |
| ALK[I1171S] | GST | ALK[I1171S] | 08-569 | AQT0794 | 15 µM | AssayQuant Technologies | |
| ALK[I1171T] | GST | ALK[I1171T] | 08-590 | AQT0794 | 15 µM | AssayQuant Technologies | |
| ALK[L1196M／G1202R] | GST | ALK[L1196M／G1202R] | 08-556 | AQT0101 | 10 µM | AssayQuant Technologies | |
| ALK[L1198F] | GST | ALK[L1198F] | 08-571 | AQT0794 | 15 µM | AssayQuant Technologies | |
| ALK[V1180L] | GST | ALK[V1180L] | 08-570 | AQT0794 | 15 µM | AssayQuant Technologies | |
| ARG(ABL2) | GST | ARG(ABL2) | 08-102 | AQT0032 | 10 µM | AssayQuant Technologies | |
| AurA | GST | AurA (AURKA) | 05-101 | AQT0186 | 15 µM | AssayQuant Technologies | |
| AurB(AURKB)／INCENP | GST | AurB(AURKB)／INCENP | 05-102 | AQT0186 | 15 µM | AssayQuant Technologies | |
| AurC | GST | AurC (AURKC) | 05-103 | AQT0186 | 15 µM | AssayQuant Technologies | |
| AXL | GST | AXL | 08-107 | AQT0794 | 15 µM | AssayQuant Technologies | |
| BLK | GST | BLK | 08-164 | AQT0794 | 15 µM | AssayQuant Technologies | |
| BMX | GST | BMX | 08-179 | AQT0794 | 15 µM | AssayQuant Technologies | |
| BRK(PTK6) | GST | BRK(PTK6) | 08-165 | AQT0794 | 15 µM | AssayQuant Technologies | |
| BRSK1 | GST | BRSK1 | 02-115 | AQT0900 | 15 µM | AssayQuant Technologies | |
| BRSK2 | GST | BRSK2 | 02-116 | AQT0900 | 15 µM | AssayQuant Technologies | |
| BTK | GST | BTK | 08-180 | AQT0101 | 15 µM | AssayQuant Technologies | |
| BUB1／BUB3 | GST | BUB1／BUB3 | 05-187 | AQT0345 | 15 µM | AssayQuant Technologies | |
| BUBR1 | GST | BUBR1 (BUB1B) | 05-105 | AQT0683 | 15 µM | AssayQuant Technologies | |
| CaMK2α(CAMK2A) | GST | CaMK2α(CAMK2A) | 02-109 | AQT0356 | 15 µM | AssayQuant Technologies | |
| CAMKK2 | GST | CAMKK2 | 05-108 | AQT1116 | 15 µM | AssayQuant Technologies | |
| CDK1(CDC2)／CycA2 | GST | CDK1(CDC2)／CycA2 | 04-119 | AQT0297 | 15 µM | AssayQuant Technologies | |
| CDK1(CDC2)／CycB1 | GST | CDK1(CDC2)／CycB1 | 04-102 | AQT0297 | 15 µM | AssayQuant Technologies | |
| CDK12(CRKRS)(720-1490aa)／CycK | BTN | BTN-CDK12(CRKRS)(720-1490aa)／CycK | 04-413-20N | AQT0991 | 15 µM | AssayQuant Technologies | |
| CDK2／CycA2 | GST | CDK2／CycA2 | 04-103 | AQT0297 | 15 µM | AssayQuant Technologies | |
| CDK2／CycE1 | GST | CDK2／CycE1 | 04-165 | AQT0297 | 15 µM | AssayQuant Technologies | |
| CDK3／CycE1 | GST | CDK3／CycE1 | 04-104 | AQT0297 | 15 µM | AssayQuant Technologies | |
| CDK4／CycD3 | GST | CDK4／CycD3 | 04-105 | AQT0258 | 15 µM | AssayQuant Technologies | |
| CDK6／CycD1 | GST | CDK6／CycD1 | 04-114 | AQT0258 | 15 µM | AssayQuant Technologies | |
| CDK6／CycD3 | GST | CDK6／CycD3 | 04-107 | AQT0258 | 15 µM | AssayQuant Technologies | |
| CDK7／CycH／MAT1 | GST | CDK7／CycH／MAT1 | 04-108 | AQT0733 | 15 µM | AssayQuant Technologies | |
| CDK9／CycK | GST | CDK9／CycK | 04-111 | AQT1007 | 15 µM | AssayQuant Technologies | |
| CDK9／CycT1 | GST | CDK9／CycT1 | 04-110 | AQT0733 | 15 µM | AssayQuant Technologies | |
| CDK9／CycT2 | GST | CDK9／CycT2 | 04-121 | AQT0733 | 15 µM | AssayQuant Technologies | |
| CHK1(CHEK1) | GST | CHK1(CHEK1) | 02-117 | AQT0694 | 15 µM | AssayQuant Technologies | |
| CHK2(CHEK2) | GST | CHK2(CHEK2) | 02-162 | AQT0694 | 15 µM | AssayQuant Technologies | |
| CK1α | GST | CK1α (CSNK1A1) | 03-101 | AQT0709 | 15 µM | AssayQuant Technologies | |
| CK1γ1 | GST | CK1γ1 (CSNK1G1) | 03-105 | AQT0709 | 15 µM | AssayQuant Technologies | |
| CK1γ2 | GST | CK1γ2 (CSNK1G2) | 03-106 | AQT0709 | 15 µM | AssayQuant Technologies | |
| CK1γ3 | GST | CK1γ3 (CSNK1G3) | 03-107 | AQT0709 | 15 µM | AssayQuant Technologies | |
| CK1δ | GST | CK1δ (CSNK1D) | 03-103 | AQT0709 | 15 µM | AssayQuant Technologies | |
| CK1ε | GST | CK1ε (CSNK1E) | 03-104 | AQT0709 | 15 µM | AssayQuant Technologies | |
| COT | Tag-free | COT (MAP3K8) | 07-301 | AQT0918 | 15 µM | AssayQuant Technologies | |
| CRIK | GST | CRIK (CIT) | 01-104 | AQT0537 | 15 µM | AssayQuant Technologies | |
| CSK | GST | CSK | 08-111 | AQT0794 | 15 µM | AssayQuant Technologies | |
| DDR1(SRC treated) | GST | DDR1(SRC treated) | 08-132 | AQT0280 | 15 µM | AssayQuant Technologies | |
| DDR2 | GST | DDR2 | 08-114 | AQT0235 | 15 µM | AssayQuant Technologies | |
| DYRK1A | GST | DYRK1A | 04-130 | AQT0260 | 20 µM | AssayQuant Technologies | |
| DYRK1B | GST | DYRK1B | 04-131 | AQT0260 | 20 µM | AssayQuant Technologies | |
| EPHA1 | GST | EPHA1 | 08-119 | AQT0658 | 15 µM | AssayQuant Technologies | |
| EPHA2 | GST | EPHA2 | 08-121 | AQT0661 | 15 µM | AssayQuant Technologies | |
| EPHA3 | GST | EPHA3 | 08-122 | AQT0661 | 15 µM | AssayQuant Technologies | |
| EPHA4 | GST | EPHA4 | 08-123 | AQT0661 | 15 µM | AssayQuant Technologies | |
| EPHA5 | GST | EPHA5 | 08-124 | AQT0658 | 15 µM | AssayQuant Technologies | |
| EPHA6 | GST | EPHA6 | 08-125 | AQT0653 | 15 µM | AssayQuant Technologies | |
| EPHA7 | GST | EPHA7 | 08-126 | AQT0653 | 15 µM | AssayQuant Technologies | |
| EPHA8 | GST | EPHA8 | 08-127 | AQT0661 | 15 µM | AssayQuant Technologies | |
| EPHB1 | GST | EPHB1 | 08-128 | AQT0661 | 15 µM | AssayQuant Technologies | |
| EPHB2 | GST | EPHB2 | 08-129 | AQT0661 | 15 µM | AssayQuant Technologies | |
| EPHB3 | GST | EPHB3 | 08-130 | AQT0661 | 15 µM | AssayQuant Technologies | |
| EPHB4 | GST | EPHB4 | 08-131 | AQT0661 | 15 µM | AssayQuant Technologies | |
| Erk1(MAPK3) | GST | Erk1(MAPK3) | 04-142 | AQT0490 | 10 µM | AssayQuant Technologies | |
| Erk2(MAPK1) | GST | Erk2(MAPK1) | 04-143 | AQT0490 | 10 µM | AssayQuant Technologies | |
| FER | GST | FER | 08-139 | AQT0794 | 15 µM | AssayQuant Technologies | |
| FES | GST | FES | 08-140 | AQT0794 | 15 µM | AssayQuant Technologies | |
| FGFR1 | GST | FGFR1 | 08-133 | AQT0794 | 15 µM | AssayQuant Technologies | |
| FGFR2 | GST | FGFR2 | 08-134 | AQT0794 | 15 µM | AssayQuant Technologies | |
| FGFR3 | GST | FGFR3 | 08-135 | AQT0794 | 15 µM | AssayQuant Technologies | |
| FGFR3[V555M] | GST | FGFR3[V555M] | 08-543 | AQT0515 | 15 µM | AssayQuant Technologies | |
| FGFR4 | GST | FGFR4 | 08-136 | AQT0794 | 15 µM | AssayQuant Technologies | |
| FGR | GST | FGR | 08-166 | AQT0661 | 15 µM | AssayQuant Technologies | |
| FLT1 | GST | FLT1 | 08-189 | AQT0794 | 15 µM | AssayQuant Technologies | |
| FLT3 | GST | FLT3 | 08-154 | AQT0794 | 15 µM | AssayQuant Technologies | |
| FLT4 | GST | FLT4 | 08-190 | AQT0794 | 15 µM | AssayQuant Technologies | |
| FMS(CSF1R) | GST | FMS(CSF1R) | 08-155 | AQT0794 | 15 µM | AssayQuant Technologies | |
| FRK | GST | FRK | 08-167 | AQT0794 | 15 µM | AssayQuant Technologies | |
| FYN [isoform a] | GST | FYN [isoform a] | 08-168 | AQT0794 | 15 µM | AssayQuant Technologies | |
| FYN [isoform b] | GST | FYN [isoform b] | 08-531 | AQT0794 | 15 µM | AssayQuant Technologies | |
| HCK | GST | HCK | 08-169 | AQT0794 | 15 µM | AssayQuant Technologies | |
| HER4 | GST | HER4 (ERBB4) | 08-118 | AQT0794 | 15 µM | AssayQuant Technologies | |
| HGK | GST | HGK (MAP4K4) | 07-137 | AQT0135 | 15 µM | AssayQuant Technologies | |
| IGF1R | GST | IGF1R | 08-141 | AQT0794 | 15 µM | AssayQuant Technologies | |
| IKKα(CHUK) | GST | IKKα(CHUK) | 05-112 | AQT0683 | 15 µM | AssayQuant Technologies | |
| INSR | GST | INSR | 08-142 | AQT0794 | 15 µM | AssayQuant Technologies | |
| IRR | GST | IRR (INSRR) | 08-143 | AQT0661 | 15 µM | AssayQuant Technologies | |
| ITK | GST | ITK | 08-181 | AQT0101 | 15 µM | AssayQuant Technologies | |
| JAK2 | HIS | JAK2 | 08-045 | AQT0661 | 15 µM | AssayQuant Technologies | |
| JAK2(JH1 JH2) | GST | JAK2(JH1 JH2) | 08-514 | AQT0661 | 15 µM | AssayQuant Technologies | |
| JAK3 | HIS | JAK3 | 08-046 | AQT0661 | 15 µM | AssayQuant Technologies | |
| KDR | GST | KDR | 08-191 | AQT0794 | 15 µM | AssayQuant Technologies | |
| KIT | GST | KIT | 08-156 | AQT0661 | 15 µM | AssayQuant Technologies | |
| KIT[D816V] | GST | KIT[D816V] | 08-505 | AQT0101 | 15 µM | AssayQuant Technologies | |
| LATS1／MOBKL1A | GST | LATS1／MOBKL1A | 01-523 | AQT0476 | 15 µM | AssayQuant Technologies | |
| LATS2／MOBKL1A | GST | LATS2／MOBKL1A | 01-524 | AQT0476 | 15 µM | AssayQuant Technologies | |
| LCK | GST | LCK | 08-170 | AQT0794 | 15 µM | AssayQuant Technologies | |
| LTK | GST | LTK | 08-106 | AQT0794 | 15 µM | AssayQuant Technologies | |
| LYNa | GST | LYNa | 08-171 | AQT0794 | 15 µM | AssayQuant Technologies | |
| LYNb | GST | LYNb | 08-172 | AQT0794 | 15 µM | AssayQuant Technologies | |
| MAP3K1 | GST | MAP3K1 | 07-103 | AQT0623 | 15 µM | AssayQuant Technologies | |
| MAP3K14 | GST | MAP3K14 | 07-102 | AQT0912 | 15 µM | AssayQuant Technologies | |
| MAPKAPK2 | GST | MAPKAPK2 | 02-142 | AQT0426 | 15 µM | AssayQuant Technologies | |
| MAPKAPK3 | GST | MAPKAPK3 | 02-143 | AQT0425 | 15 µM | AssayQuant Technologies | |
| MAPKAPK5 | GST | MAPKAPK5 | 02-144 | AQT0426 | 15 µM | AssayQuant Technologies | |
| MAPKAPK5 | GST | MAPKAPK5 | 02-144 | AQT1102 | 15 µM | AssayQuant Technologies | |
| MER(MERTK) | GST | MER(MERTK) | 08-108 | AQT0794 | 15 µM | AssayQuant Technologies | |
| MET | GST | MET | 08-151 | AQT0658 | 15 µM | AssayQuant Technologies | |
| MET[D1228N] | GST | MET[D1228N] | 08-120 | AQT0702 | 15 µM | AssayQuant Technologies | |
| MET[Y1230H] | GST | MET[Y1230H] | 08-178 | AQT0658 | 15 µM | AssayQuant Technologies | |
| MLK2(MAP3K10) | GST | MLK2(MAP3K10) | 09-116 | AQT0646 | 15 µM | AssayQuant Technologies | |
| MLK3(MAP3K11) | GST | MLK3(MAP3K11) | 09-117 | AQT0646 | 15 µM | AssayQuant Technologies | |
| MNK1(MKNK1) | GST | MNK1(MKNK1) | 02-145 | AQT0414 | 15 µM | AssayQuant Technologies | |
| MNK2 | GST | MNK2 (MKNK2) | 02-146 | AQT0701 | 15 µM | AssayQuant Technologies | |
| MRCKβ | GST | MRCKβ (CDC42BPB) | 01-108 | AQT0837 | 15 µM | AssayQuant Technologies | |
| MSK1 | GST | MSK1 (RPS6KA5) | 01-147 | AQT0797 | 10 µM | AssayQuant Technologies | |
| MSK2 | GST | MSK2 (RPS6KA4) | 01-148 | AQT0988 | 15 µM | AssayQuant Technologies | |
| MTOR(FRAP)／MLST8 | DYKDDDDK | MTOR(FRAP)／MLST8 | 11-431 | AQT0732 | 20 µM | AssayQuant Technologies | |
| MUSK | GST | MUSK | 08-153 | AQT0031 | 15 µM | AssayQuant Technologies | |
| NEK3 | GST | NEK3 | 05-127 | AQT0685 | 15 µM | AssayQuant Technologies | |
| NEK6 | GST | NEK6 | 05-130 | AQT0688 | 15 µM | AssayQuant Technologies | |
| NEK7 | GST | NEK7 | 05-131 | AQT0887 | 15 µM | AssayQuant Technologies | |
| p38α(MAPK14) | GST | p38α(MAPK14) | 04-152 | AQT0490 | 10 µM | AssayQuant Technologies | |
| p38β | GST | p38β (MAPK11) | 04-153 | AQT0490 | 10 µM | AssayQuant Technologies | |
| p38γ | GST | p38γ (MAPK12) | 04-155 | AQT0652 | 10 µM | AssayQuant Technologies | |
| p38δ | GST | p38δ (MAPK13) | 04-154 | AQT0652 | 10 µM | AssayQuant Technologies | |
| PASK | GST | PASK | 02-128 | AQT0694 | 15 µM | AssayQuant Technologies | |
| PCTAIRE1(CDK16)／CycY | GST | PCTAIRE1(CDK16)／CycY | 04-116 | AQT0258 | 15 µM | AssayQuant Technologies | |
| PDGFRα(PDGFRA) | GST | PDGFRα(PDGFRA) | 08-157 | AQT0661 | 15 µM | AssayQuant Technologies | |
| PDGFRα(PDGFRA)[V561D] | GST | PDGFRα(PDGFRA)[V561D] | 08-507 | AQT0734 | 15 µM | AssayQuant Technologies | |
| PDGFRβ(PDGFRB) | GST | PDGFRβ(PDGFRB) | 08-158 | AQT0661 | 15 µM | AssayQuant Technologies | |
| PIM1 | HIS | PIM1 | 02-054 | AQT0474 | 15 µM | AssayQuant Technologies | |
| PIM2 | GST | PIM2 | 02-155 | AQT0474 | 15 µM | AssayQuant Technologies | |
| PIM3 | GST | PIM3 | 02-156 | AQT0474 | 15 µM | AssayQuant Technologies | |
| PKACα(PRKACA) | GST | PKACα(PRKACA) | 01-127 | AQT0458 | 15 µM | AssayQuant Technologies | |
| PKACβ(PRKACB) | GST | PKACβ(PRKACB) | 01-128 | AQT0458 | 15 µM | AssayQuant Technologies | |
| PKD1 | GST | PKD1 (PRKD1) | 02-157 | AQT0803 | 15 µM | AssayQuant Technologies | |
| PKD2 | GST | PKD2 (PRKD2) | 02-158 | AQT0803 | 15 µM | AssayQuant Technologies | |
| PKD3 | GST | PKD3 (PRKD3) | 02-159 | AQT0803 | 15 µM | AssayQuant Technologies | |
| PRKX | GST | PRKX | 01-130 | AQT0186 | 15 µM | AssayQuant Technologies | |
| PYK2(PTK2B) | GST | PYK2(PTK2B) | 08-138 | AQT0661 | 15 µM | AssayQuant Technologies | |
| RET | GST | RET | 08-159 | AQT0661 | 15 µM | AssayQuant Technologies | |
| RET[G810C／M918T] | GST | RET[G810C／M918T] | 08-580 | AQT0101 | 15 µM | AssayQuant Technologies | |
| RET[V804M] | BTN | BTN-RET[V804M] * | 08-460-20N | AQT0101 | 15 µM | AssayQuant Technologies | |
| ROCK1 | GST | ROCK1 | 01-109 | AQT0839 | 15 µM | AssayQuant Technologies | |
| ROCK2 | GST | ROCK2 | 01-110 | AQT0839 | 15 µM | AssayQuant Technologies | |
| RON | GST | RON (MST1R) | 08-152 | AQT0794 | 15 µM | AssayQuant Technologies | |
| ROS(ROS1) | GST | ROS(ROS1) | 08-163 | AQT0661 | 15 µM | AssayQuant Technologies | |
| ROS(ROS1)[G2032R／L2086F] | GST | ROS(ROS1)[G2032R／L2086F] | 08-587 | AQT0101 | 15 µM | AssayQuant Technologies | |
| RSK1(RPS6KA1) | GST | RSK1(RPS6KA1) | 01-149 | AQT0232 | 15 µM | AssayQuant Technologies | |
| RSK2(RPS6KA3) | GST | RSK2(RPS6KA3) | 01-150 | AQT0232 | 15 µM | AssayQuant Technologies | |
| RSK3(RPS6KA2) | GST | RSK3(RPS6KA2) | 01-151 | AQT0232 | 15 µM | AssayQuant Technologies | |
| RSK4(RPS6KA6) | GST | RSK4(RPS6KA6) | 01-152 | AQT0232 | 15 µM | AssayQuant Technologies | |
| SGK | GST | SGK | 01-158 | AQT0536 | 15 µM | AssayQuant Technologies | |
| SGK2 | GST | SGK2 | 01-159 | AQT0536 | 15 µM | AssayQuant Technologies | |
| SGK3 | GST | SGK3 (SGKL) | 01-160 | AQT0536 | 15 µM | AssayQuant Technologies | |
| SRC | GST | SRC | 08-173 | AQT0794 | 15 µM | AssayQuant Technologies | |
| SRM | GST | SRM (SRMS) | 08-174 | AQT0794 | 15 µM | AssayQuant Technologies | |
| SYK | GST | SYK | 08-176 | AQT0794 | 15 µM | AssayQuant Technologies | |
| TAK1-TAB1(MAP3K7) | BTN | BTN-TAK1-TAB1(MAP3K7) * | 09-419-21C | AQT0356 | 15 µM | AssayQuant Technologies | |
| TAOK2 | GST | TAOK2 | 07-133 | AQT0253 | 15 µM | AssayQuant Technologies | |
| TBK1 | GST | TBK1 | 05-115 | AQT0897 | 15 µM | AssayQuant Technologies | |
| TEC | GST | TEC | 08-182 | AQT0794 | 15 µM | AssayQuant Technologies | |
| TIE2(TEK) | GST | TIE2(TEK) | 08-185 | AQT0794 | 15 µM | AssayQuant Technologies | |
| TLK1 | GST | TLK1 | 05-166 | AQT0686 | 15 µM | AssayQuant Technologies | |
| TLK2 | GST | TLK2 | 05-167 | AQT0686 | 15 µM | AssayQuant Technologies | |
| TNIK | GST | TNIK | 07-138 | AQT0135 | 15 µM | AssayQuant Technologies | |
| TRKA(NTRK1) | GST | TRKA(NTRK1) | 08-186 | AQT0794 | 15 µM | AssayQuant Technologies | |
| TRKB(NTRK2) | GST | TRKB(NTRK2) | 08-187 | AQT0794 | 15 µM | AssayQuant Technologies | |
| TRKC(NTRK3) | GST | TRKC(NTRK3) | 08-197 | AQT0794 | 15 µM | AssayQuant Technologies | |
| TTBK2 | GST | TTBK2 | 03-109 | AQT0686 | 15 µM | AssayQuant Technologies | |
| TTK | GST | TTK | 05-169 | AQT0897 | 15 µM | AssayQuant Technologies | |
| TXK | GST | TXK | 08-183 | AQT0794 | 15 µM | AssayQuant Technologies | |
| TYK2 | GST | TYK2 | 08-147 | AQT0663 | 20 µM | AssayQuant Technologies | |
| TYRO3 | GST | TYRO3 | 08-109 | AQT0794 | 15 µM | AssayQuant Technologies | |
| ULK3 | GST | ULK3 | 05-173 | AQT0686 | 15 µM | AssayQuant Technologies | |
| WEE1 | BTN | BTN-WEE1 | 05-477-20N | AQT0728 | 40 µM | AssayQuant Technologies | |
| YES(YES1) | GST | YES(YES1) | 08-175 | AQT0794 | 15 µM | AssayQuant Technologies | |
| ZAK | GST | ZAK | 09-120 | AQT0753 | 15 µM | AssayQuant Technologies | |
| ZAP70 | GST | ZAP70 | 08-177 | AQT0794 | 15 µM | AssayQuant Technologies | |
PhosphoSens™アッセイ原理＆アッセイフォーマット
PhosphoSens™ ではSoxをシステイン残基に導入した改良型CSoxセンサーを使用。CSoxを結合させた基質がキナーゼ、ATP、Mg2+の存在下でリン酸化されるとMg2+がリン酸基とCSoxにキレーティングされることにより複合体が形成され、ここに励起光を照射することで蛍光を発する。この蛍光強度の変化を経時的に検出することで、基質のリン酸化状態の変化をリアルタイムでモニターすることが出来る。
（本技術はマサチューセッツ工科大学（MIT)にて開発、MITが保持する特許を独占的にAssayQuant社が実施）
（本技術はマサチューセッツ工科大学（MIT)にて開発、MITが保持する特許を独占的にAssayQuant社が実施）
- PhosphoSens‑Kineticフォーマット（連続蛍光強度測定）
- 精製されたキナーゼ酵素やその他サンプル（例：細胞溶解液、組織ホモジネート）を用いて、IC₅₀、作用機序（MOA/MOI）、構造活性相関（SAR）研究に必要なリアルタイムでのキネティックス測定が可能です。
- PhosphoSens‑Red（時間分解蛍光、エンドポイント測定）
- ハイスループットスクリーニング（HTS）に最適なTRF（時間分解蛍光）ベースのフォーマットで、化合物の自己蛍光を最小限に抑えつつ、PhosphoSens技術を最大限に活かした測定が可能です。
- PhosphoSens™技術を用いたキット製品の購入、アッセイ試験の外部委託をご検討されている方へ
- AssayQuant Technologies社提供 アッセイキット製品・サービスについては下のリンクより各専用サイトで詳細情報をご覧頂けます。ご購入・ご利用等のご相談につきましてはお問い合わせください。
