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Mobility Shift Assay(MSA)/IMAP™ Kinase Assay Preincubation Targets

It's known that some kinase inhibitors which have strong potency are slow binders to the targets. This Preincubation Study Service measures and determines true activity of slow binders by incorporating a 30 minute preincubation at room temperature with the test compound(s).

Please check study schedule and assay conditions, and use the application form for Preincubation Assay. Submit the completed application form as an e-mail attachment at info@carnabio.com.
If you prefer to select from other panels, ALL targets, please start from here.

Target Name Group ATP conc. Source
(Carna Cat. No.)
ABL(ABL1) Cytoplasmic Tyrosine Km bin./1mM 08-001
ABL(ABL1)[E255K] Cytoplasmic Tyrosine Km bin./1mM 08-094
ABL(ABL1)[T315I] Cytoplasmic Tyrosine Km bin./1mM 08-093
AKT1 Serine / Threonine Km bin./1mM 01-101
AKT2 Serine / Threonine Km bin. 01-102
AKT3 Serine / Threonine Km bin. 01-103
ALK Receptor Tyrosine Km bin./1mM 08-518
ALK[C1156Y] Receptor Tyrosine Km bin./1mM 08-530
ALK[F1174L] Receptor Tyrosine Km bin./1mM 08-519
ALK[G1202R] Receptor Tyrosine Km bin./1mM 08-544
ALK[G1269A] Receptor Tyrosine Km bin./1mM 08-537
ALK[L1196M] Receptor Tyrosine Km bin./1mM 08-529
ALK[R1275Q] Receptor Tyrosine Km bin./1mM 08-520
AMPKα1/β1/γ1 (PRKAA1/B1/G1) Serine / Threonine Km bin./1mM 02-113
AMPKα2/β1/γ1 Serine / Threonine Km bin. 02-114
ARG(ABL2) Cytoplasmic Tyrosine Km bin./1mM 08-102
AurA (AURKA) Serine / Threonine Km bin./1mM 05-101
AurA(AURKA)/TPX2 Serine / Threonine Km bin. 05-186
AurB(AURKB)/INCENP Serine / Threonine Km bin./1mM 05-102
BMX Cytoplasmic Tyrosine Km bin./1mM 08-179
BRSK1 Serine / Threonine Km bin./1mM 02-115
BRSK2 Serine / Threonine Km bin. 02-116
BUB1/BUB3 Serine / Threonine Km bin. 05-187
CaMK2α(CAMK2A) Serine / Threonine Km bin. 02-109
CaMK2β(CAMK2B) Serine / Threonine Km bin. 02-110
CaMK2γ Serine / Threonine Km bin. 02-112
CaMK2δ Serine / Threonine Km bin. 02-111
CaMK4 Serine / Threonine Km bin./1mM 02-108
CDC2/CycB1 Serine / Threonine Km bin./1mM 04-102
CDK2/CycA2 Serine / Threonine Km bin./1mM 04-103
CDK2/CycE1 Serine / Threonine Km bin./1mM 04-165
CDK3/CycE1 Serine / Threonine Km bin. 04-104
CDK5/p25 Serine / Threonine Km bin./1mM 04-106
CGK2 Serine / Threonine Km bin. 01-143
CHK1(CHEK1) Serine / Threonine Km bin./1mM 02-117
CHK2(CHEK2) Serine / Threonine Km bin./1mM 02-162
CK1γ1 Serine / Threonine Km bin. 03-105
CK1γ2 Serine / Threonine Km bin. 03-106
CK1γ3 Serine / Threonine Km bin. 03-107
CK1δ Serine / Threonine Km bin. 03-103
CK2α1/β (CSNK2A1) Serine / Threonine Km bin./1mM 05-184
CK2α2/β Serine / Threonine Km bin. 05-185
CLK1 Serine / Threonine Km bin./1mM 04-126
CLK2 Serine / Threonine Km bin./1mM 04-127
CLK3 Serine / Threonine Km bin. 04-128
DAPK1 Serine / Threonine Km bin./1mM 02-134
DYRK1A Serine / Threonine Km bin./1mM 04-130
DYRK1B Serine / Threonine Km bin./1mM 04-131
DYRK2 Serine / Threonine Km bin. 04-132
DYRK3 Serine / Threonine Km bin. 04-133
EGFR (ERBB1) Receptor Tyrosine Km bin./1mM 08-115
EGFR[d746-750/T790M] (ERBB1) Receptor Tyrosine Km bin./1mM 08-528
EGFR[d746-750] (ERBB1) Receptor Tyrosine Km bin./1mM 08-527
EGFR[d746-750/C797S] NEW Receptor Tyrosine Km bin./1mM 08-564
EGFR[L858R] (ERBB1) Receptor Tyrosine Km bin./1mM 08-502
EGFR[L861Q] (ERBB1) Receptor Tyrosine Km bin./1mM 08-513
EGFR[T790M] (ERBB1) Receptor Tyrosine Km bin./1mM 08-194
EGFR[T790M/L858R] (ERBB1) Receptor Tyrosine Km bin./1mM 08-510
EPHA1 Receptor Tyrosine Km bin./1mM 08-119
EPHA2 Receptor Tyrosine Km bin./1mM 08-121
EPHA3 Receptor Tyrosine Km bin./1mM 08-122
EPHA4 Receptor Tyrosine Km bin./1mM 08-123
EPHA5 Receptor Tyrosine Km bin./1mM 08-124
EPHA6 Receptor Tyrosine Km bin./1mM 08-125
EPHA7 Receptor Tyrosine Km bin./1mM 08-126
EPHA8 Receptor Tyrosine Km bin./1mM 08-127
EPHB1 Receptor Tyrosine Km bin./1mM 08-128
EPHB2 Receptor Tyrosine Km bin./1mM 08-129
EPHB3 Receptor Tyrosine Km bin./1mM 08-130
EPHB4 Receptor Tyrosine Km bin./1mM 08-131
Erk1(MAPK3) Serine / Threonine Km bin./1mM 04-142
Erk2(MAPK1) Serine / Threonine Km bin./1mM 04-143
FER Cytoplasmic Tyrosine Km bin./1mM 08-139
FES Cytoplasmic Tyrosine Km bin./1mM 08-140
FGFR1 Receptor Tyrosine Km bin./1mM 08-133
FGFR2 Receptor Tyrosine Km bin./1mM 08-134
FGFR2[V564I] Receptor Tyrosine Km bin./1mM 08-546
FGFR4 Receptor Tyrosine Km bin./1mM 08-136
FGFR4[V550L] Receptor Tyrosine Km bin./1mM 08-526
FGR Cytoplasmic Tyrosine Km bin./1mM 08-166
FLT1 Receptor Tyrosine Km bin./1mM 08-189
FLT4 Receptor Tyrosine Km bin./1mM 08-190
FRK Cytoplasmic Tyrosine Km bin./1mM 08-167
GSK3α Serine / Threonine Km bin./1mM 04-140
GSK3β(GSK3B) Serine / Threonine Km bin./1mM 04-141
Haspin Serine / Threonine Km bin. 05-111
HER4 (ERBB4) Receptor Tyrosine Km bin./1mM 08-118
HIPK1 Serine / Threonine Km bin. 04-135
HIPK2 Serine / Threonine Km bin. 04-136
HIPK3 Serine / Threonine Km bin. 04-137
HIPK4 Serine / Threonine Km bin./1mM 04-138
IGF1R Receptor Tyrosine Km bin./1mM 08-141
IKKβ (IKBKB) Serine / Threonine Km bin./1mM 05-084
INSR Receptor Tyrosine Km bin./1mM 08-142
JAK2 Cytoplasmic Tyrosine Km bin./1mM 08-045
JAK3 Cytoplasmic Tyrosine Km bin./1mM 08-046
JNK1(MAPK8) Serine / Threonine Km bin./1mM 04-163
JNK2(MAPK9) Serine / Threonine Km bin./1mM 04-164
JNK3 (MAPK10) Serine / Threonine Km bin./1mM 04-150
KDR(VEGFR2) Receptor Tyrosine Km bin./1mM 08-191
LCK Cytoplasmic Tyrosine Km bin./1mM 08-170
LYNa Cytoplasmic Tyrosine Km bin./1mM 08-171
LYNb Cytoplasmic Tyrosine Km bin./1mM 08-172
MAPKAPK2 Serine / Threonine Km bin./1mM 02-142
MAPKAPK3 Serine / Threonine Km bin. 02-143
MAPKAPK5 Serine / Threonine Km bin. 02-144
MARK1 Serine / Threonine Km bin. 02-120
MARK2 Serine / Threonine Km bin. 02-121
MARK3 Serine / Threonine Km bin. 02-122
MARK4 Serine / Threonine Km bin. 02-123
MER(MERTK) Receptor Tyrosine Km bin./1mM 08-108
MET Receptor Tyrosine Km bin./1mM 08-151
MET[D1228H] Receptor Tyrosine Km bin./1mM 08-540
MET[M1250T] Receptor Tyrosine Km bin./1mM 08-545
MET[Y1235D] Receptor Tyrosine Km bin./1mM 08-198
MNK1 Serine / Threonine Km bin. 02-145
MNK2 Serine / Threonine Km bin. 02-146
MSK1 Serine / Threonine Km bin. 01-147
NEK2 Serine / Threonine Km bin./1mM 05-226
NEK4 Serine / Threonine Km bin. 05-128
NIM1K(MGC42105) Serine / Threonine Km bin. 02-175
NPM1-ALK Receptor Tyrosine Km bin./1mM 08-517
NuaK1(ARK5) Serine / Threonine Km bin. 02-126
NuaK2 Serine / Threonine Km bin. 02-127
p38α(MAPK14) Serine / Threonine Km bin./1mM 04-152
p38β (MAPK11) Serine / Threonine Km bin./1mM 04-153
p38γ (MAPK12) Serine / Threonine Km bin./1mM 04-155
p38δ (MAPK13) Serine / Threonine Km bin./1mM 04-154
p70S6K(RPS6KB1) Serine / Threonine Km bin./1mM 01-154
p70S6Kβ(RPS6KB2) Serine / Threonine Km bin. 01-155
PAK1 Serine / Threonine Km bin. 07-123
PAK2 Serine / Threonine Km bin./1mM 07-124
PAK5 Serine / Threonine Km bin. 07-127
PDGFRα(PDGFRA) Receptor Tyrosine Km bin./1mM 08-157
PDGFRα(PDGFRA)[D842V] Receptor Tyrosine Km bin./1mM 08-506
PDGFRα(PDGFRA)[V561D] Receptor Tyrosine Km bin./1mM 08-507
PDGFRβ(PDGFRB) Receptor Tyrosine Km bin./1mM 08-158
PIM1 Serine / Threonine Km bin./1mM 02-054
PKCδ(PRKCD) Serine / Threonine Km bin. 01-135
PKCε(PRKCE) Serine / Threonine Km bin./1mM 01-136
PKCζ Serine / Threonine Km bin. 01-141
PKCη Serine / Threonine Km bin. 01-138
PKD1 Serine / Threonine Km bin. 02-157
PKD2 (PRKD2) Serine / Threonine Km bin./1mM 02-158
PYK2(PTK2B) Cytoplasmic Tyrosine Km bin./1mM 08-138
QIK (SIK2) Serine / Threonine Km bin./1mM 02-129
RET Receptor Tyrosine Km bin./1mM 08-159
RET[G691S] Receptor Tyrosine Km bin./1mM 08-522
RET[M918T] Receptor Tyrosine Km bin./1mM 08-508
RET[S891A] Receptor Tyrosine Km bin./1mM 08-523
RET[Y791F] Receptor Tyrosine Km bin./1mM 08-521
ROCK2 Serine / Threonine Km bin. 01-110
RON (MST1R) Receptor Tyrosine Km bin./1mM 08-152
ROS (ROS1) Receptor Tyrosine Km bin./1mM 08-163
RSK2(RPS6KA3) Serine / Threonine Km bin. 01-150
RSK3(RPS6KA2) Serine / Threonine Km bin./1mM 01-151
RSK4(RPS6KA6) Serine / Threonine Km bin./1mM 01-152
SGK Serine / Threonine Km bin./1mM 01-158
SGK3 Serine / Threonine Km bin. 01-160
skMLCK Serine / Threonine Km bin. 02-150
SPHK1 Lipid Km bin. 11-105
SRC Cytoplasmic Tyrosine Km bin./1mM 08-173
SRM (SRMS) Cytoplasmic Tyrosine Km bin./1mM 08-174
TBK1 Serine / Threonine Km bin. 05-115
TEC Cytoplasmic Tyrosine Km bin./1mM 08-182
TIE2(TEK) Receptor Tyrosine Km bin./1mM 08-185
TRKB(NTRK2) Receptor Tyrosine Km bin./1mM 08-187
TRKC(NTRK3) Receptor Tyrosine Km bin./1mM 08-197
TSSK1 Serine / Threonine Km bin./1mM 02-364
TYRO3 Receptor Tyrosine Km bin./1mM 08-109
YES(YES1) Cytoplasmic Tyrosine Km bin./1mM 08-175
YES(YES1)[T348I] Cytoplasmic Tyrosine Km bin./1mM 08-533

Preincubation Study Methodology

Preincubation Study Methodology

Reference Data

IC50 Comparison with or without Preincubation for p38α Inhibitors
IC50 Comparison with or without Preincubation for Clinical Inhibitors

Service Schedule

November 2021
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December 2021
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Test sample cutoff for next assays
Assay Start Day for MSA / IMAPTM
Assay Start Day for ADP-Glo™ and MSA(Preincubation)
Date in Red: Japan Holidays

Detailed Assay Conditions
for Each Kinase

Check out these & more in our Kinase Profiling Book!

Kinase Profiling Book