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- Mobility Shift Assay(MSA)/IMAP™ Kinase Assay Preincubation Targets
Mobility Shift Assay(MSA)/IMAP™ Kinase Assay Preincubation Targets
It's known that some kinase inhibitors which have strong potency are slow binders to the targets. This Preincubation IC50 Determination Study Service measures and determines true activity of slow binders by incorporating a 30 minute preincubation at room temperature with the test compound(s).
Guide for our service customers
Please check study schedule and assay conditions, and use the application form for Preincubation Assay. Submit the completed application form as an e-mail attachment at info@carnabio.com.
If you prefer to select from other panels, ALL targets, please start from here.
| Target Name | Group | ATP conc. |
Source (Carna Cat. No.) |
| ABL(ABL1) | Cytoplasmic Tyrosine | Km bin./1mM | 08-001 |
| ABL(ABL1)[E255K] | Cytoplasmic Tyrosine | Km bin./1mM | 08-094 |
| ABL(ABL1)[T315I] | Cytoplasmic Tyrosine | Km bin./1mM | 08-093 |
| AKT1 | Serine / Threonine | Km bin./1mM | 01-101 |
| AKT2 | Serine / Threonine | Km bin. | 01-102 |
| AKT3 | Serine / Threonine | Km bin. | 01-103 |
| ALK | Receptor Tyrosine | Km bin./1mM | 08-518 |
| ALK[C1156Y] | Receptor Tyrosine | Km bin./1mM | 08-530 |
| ALK[F1174L] | Receptor Tyrosine | Km bin./1mM | 08-519 |
| ALK[G1202R] | Receptor Tyrosine | Km bin./1mM | 08-544 |
| ALK[G1269A] | Receptor Tyrosine | Km bin./1mM | 08-537 |
| ALK[L1196M] | Receptor Tyrosine | Km bin./1mM | 08-529 |
| ALK[R1275Q] | Receptor Tyrosine | Km bin./1mM | 08-520 |
| AMPKα1/β1/γ1 (PRKAA1/B1/G1) | Serine / Threonine | Km bin./1mM | 02-113 |
| AMPKα2/β1/γ1 | Serine / Threonine | Km bin. | 02-114 |
| ARG(ABL2) | Cytoplasmic Tyrosine | Km bin./1mM | 08-102 |
| BMX | Cytoplasmic Tyrosine | Km bin./1mM | 08-179 |
| BRSK1 | Serine / Threonine | Km bin./1mM | 02-115 |
| BRSK2 | Serine / Threonine | Km bin. | 02-116 |
| BUB1／BUB3 | Serine / Threonine | Km bin. | 05-187 |
| CaMK2α(CAMK2A) | Serine / Threonine | Km bin./1mM Km bin. | 02-109 |
| CaMK2β(CAMK2B) | Serine / Threonine | Km bin./1mM | 02-110 |
| CaMK2γ(CAMK2G) | Serine / Threonine | Km bin./1mM Km bin. | 02-112 |
| CaMK2δ(CAMK2D) | Serine / Threonine | Km bin./1mM Km bin. | 02-111 |
| CaMK4 | Serine / Threonine | Km bin./1mM | 02-108 |
| CDK1(CDC2)／CycB1 | Serine / Threonine | Km bin./1mM | 04-102 |
| CDK2／CycA2 | Serine / Threonine | Km bin./1mM | 04-103 |
| CDK2／CycE1 | Serine / Threonine | Km bin./1mM | 04-165 |
| CDK5／p25 | Serine / Threonine | Km bin./1mM | 04-106 |
| CGK2 | Serine / Threonine | Km bin. | 01-143 |
| CHK1(CHEK1) | Serine / Threonine | Km bin./1mM | 02-117 |
| CHK2(CHEK2) | Serine / Threonine | Km bin./1mM | 02-162 |
| CK1γ1 (CSNK1G1) | Serine / Threonine | Km bin./1mM Km bin. | 03-105 |
| CK1γ2 (CSNK1G2) | Serine / Threonine | Km bin./1mM Km bin. | 03-106 |
| CK1γ3 (CSNK1G3) | Serine / Threonine | Km bin./1mM Km bin. | 03-107 |
| CK1δ | Serine / Threonine | Km bin./1mM | 03-103 |
| CK2α1/β (CSNK2A1) | Serine / Threonine | Km bin./1mM | 05-184 |
| CK2α2/β (CSNK2A2/B) | Serine / Threonine | Km bin./1mM Km bin. | 05-185 |
| CLK1 | Serine / Threonine | Km bin./1mM | 04-126 |
| CLK2 | Serine / Threonine | Km bin./1mM | 04-127 |
| CLK3 | Serine / Threonine | Km bin. | 04-128 |
| DYRK1A | Serine / Threonine | Km bin./1mM | 04-130 |
| DYRK1B | Serine / Threonine | Km bin./1mM | 04-131 |
| DYRK2 | Serine / Threonine | Km bin./1mM | 04-132 |
| DYRK3 | Serine / Threonine | Km bin./1mM | 04-133 |
| EGFR (ERBB1) | Receptor Tyrosine | Km bin./1mM | 08-115 |
| EGFR[d746-750／C797S] | Receptor Tyrosine | Km bin./1mM | 08-564 |
| EGFR[T790M／L858R] (ERBB1) | Receptor Tyrosine | Km bin./1mM | 08-510 |
| EPHA1 | Receptor Tyrosine | Km bin./1mM | 08-119 |
| EPHA2 | Receptor Tyrosine | Km bin./1mM | 08-121 |
| EPHA3 | Receptor Tyrosine | Km bin./1mM | 08-122 |
| EPHA4 | Receptor Tyrosine | Km bin./1mM | 08-123 |
| EPHA5 | Receptor Tyrosine | Km bin./1mM | 08-124 |
| EPHA6 | Receptor Tyrosine | Km bin./1mM | 08-125 |
| EPHA7 | Receptor Tyrosine | Km bin./1mM | 08-126 |
| EPHA8 | Receptor Tyrosine | Km bin./1mM | 08-127 |
| EPHB1 | Receptor Tyrosine | Km bin./1mM | 08-128 |
| EPHB2 | Receptor Tyrosine | Km bin./1mM | 08-129 |
| EPHB3 | Receptor Tyrosine | Km bin./1mM | 08-130 |
| EPHB4 | Receptor Tyrosine | Km bin./1mM | 08-131 |
| Erk1(MAPK3) | Serine / Threonine | Km bin./1mM | 04-142 |
| Erk2(MAPK1) | Serine / Threonine | Km bin./1mM | 04-143 |
| FER | Cytoplasmic Tyrosine | Km bin./1mM | 08-139 |
| FES | Cytoplasmic Tyrosine | Km bin./1mM | 08-140 |
| FGFR1 | Receptor Tyrosine | Km bin./1mM | 08-133 |
| FGFR2[V564I] | Receptor Tyrosine | Km bin./1mM | 08-546 |
| FGFR4 | Receptor Tyrosine | Km bin./1mM | 08-136 |
| FGFR4[V550L] | Receptor Tyrosine | Km bin./1mM | 08-526 |
| FLT1 | Receptor Tyrosine | Km bin./1mM | 08-189 |
| FLT4 | Receptor Tyrosine | Km bin./1mM | 08-190 |
| FRK | Cytoplasmic Tyrosine | Km bin./1mM | 08-167 |
| Haspin | Serine / Threonine | Km bin. | 05-111 |
| HER4 (ERBB4) | Receptor Tyrosine | Km bin./1mM | 08-118 |
| HIPK1 | Serine / Threonine | Km bin. | 04-135 |
| HIPK2 | Serine / Threonine | Km bin. | 04-136 |
| HIPK3 | Serine / Threonine | Km bin./1mM | 04-137 |
| IKKβ (IKBKB) | Serine / Threonine | Km bin./1mM | 05-084 |
| INSR | Receptor Tyrosine | Km bin./1mM | 08-142 |
| JAK2 | Cytoplasmic Tyrosine | Km bin./1mM | 08-045 |
| JNK1(MAPK8) | Serine / Threonine | Km bin./1mM | 04-163 |
| JNK2(MAPK9) | Serine / Threonine | Km bin./1mM | 04-164 |
| JNK3 (MAPK10) | Serine / Threonine | Km bin./1mM | 04-150 |
| KDR(VEGFR2) | Receptor Tyrosine | Km bin./1mM | 08-191 |
| LCK | Cytoplasmic Tyrosine | Km bin./1mM | 08-170 |
| LYNa | Cytoplasmic Tyrosine | Km bin./1mM | 08-171 |
| LYNb | Cytoplasmic Tyrosine | Km bin./1mM | 08-172 |
| MAP4K5(KHS1) | Serine / Threonine | Km bin./1mM Km bin. | 07-113 |
| MAPKAPK3 | Serine / Threonine | Km bin. | 02-143 |
| MAPKAPK5 | Serine / Threonine | Km bin. | 02-144 |
| MARK1 | Serine / Threonine | Km bin. | 02-120 |
| MARK3 | Serine / Threonine | Km bin. | 02-122 |
| MARK4 | Serine / Threonine | Km bin./1mM | 02-123 |
| MER(MERTK) | Receptor Tyrosine | Km bin./1mM | 08-108 |
| MET | Receptor Tyrosine | Km bin./1mM | 08-151 |
| MET[D1228H] | Receptor Tyrosine | Km bin./1mM | 08-540 |
| MET[M1250T] | Receptor Tyrosine | Km bin./1mM | 08-545 |
| MET[Y1235D] | Receptor Tyrosine | Km bin./1mM | 08-198 |
| MNK1(MKNK1) | Serine / Threonine | Km bin. | 02-145 |
| MNK2 | Serine / Threonine | Km bin./1mM | 02-146 |
| MSK1 | Serine / Threonine | Km bin./1mM Km bin. | 01-147 |
| NEK2 | Serine / Threonine | Km bin./1mM | 05-226 |
| NEK4 | Serine / Threonine | Km bin. | 05-128 |
| NIM1K(MGC42105) | Serine / Threonine | Km bin. | 02-175 |
| NPM1-ALK | Receptor Tyrosine | Km bin./1mM | 08-517 |
| NuaK1(ARK5) | Serine / Threonine | Km bin./1mM | 02-126 |
| NuaK2 | Serine / Threonine | Km bin./1mM | 02-127 |
| p38α(MAPK14) | Serine / Threonine | Km bin./1mM | 04-152 |
| p38β (MAPK11) | Serine / Threonine | Km bin./1mM | 04-153 |
| p38γ (MAPK12) | Serine / Threonine | Km bin./1mM | 04-155 |
| p38δ (MAPK13) | Serine / Threonine | Km bin./1mM | 04-154 |
| p70S6K(RPS6KB1) | Serine / Threonine | Km bin./1mM | 01-154 |
| p70S6Kβ(RPS6KB2) | Serine / Threonine | Km bin. | 01-155 |
| PAK1 | Serine / Threonine | Km bin./1mM | 07-123 |
| PAK2 | Serine / Threonine | Km bin./1mM | 07-124 |
| PAK5 | Serine / Threonine | Km bin./1mM | 07-127 |
| PDGFRα(PDGFRA) | Receptor Tyrosine | Km bin./1mM | 08-157 |
| PDGFRα(PDGFRA)[D842V] | Receptor Tyrosine | Km bin./1mM | 08-506 |
| PDGFRβ(PDGFRB) | Receptor Tyrosine | Km bin./1mM | 08-158 |
| PIM1 | Serine / Threonine | Km bin./1mM | 02-054 |
| PKCε(PRKCE) | Serine / Threonine | Km bin./1mM | 01-136 |
| PKD1 | Serine / Threonine | Km bin. | 02-157 |
| PKD2 (PRKD2) | Serine / Threonine | Km bin./1mM | 02-158 |
| PYK2(PTK2B) | Cytoplasmic Tyrosine | Km bin./1mM | 08-138 |
| QIK (SIK2) | Serine / Threonine | Km bin./1mM | 02-129 |
| RET | Receptor Tyrosine | Km bin./1mM | 08-159 |
| RET[G691S] | Receptor Tyrosine | Km bin./1mM | 08-522 |
| RET[M918T] | Receptor Tyrosine | Km bin./1mM | 08-508 |
| RET[S891A] | Receptor Tyrosine | Km bin./1mM | 08-523 |
| RET[Y791F] | Receptor Tyrosine | Km bin./1mM | 08-521 |
| RON (MST1R) | Receptor Tyrosine | Km bin./1mM | 08-152 |
| ROS(ROS1) | Receptor Tyrosine | Km bin./1mM | 08-163 |
| SGK | Serine / Threonine | Km bin./1mM | 01-158 |
| SGK3 | Serine / Threonine | Km bin. | 01-160 |
| skMLCK | Serine / Threonine | Km bin. | 02-150 |
| SRC | Cytoplasmic Tyrosine | Km bin./1mM | 08-173 |
| TBK1 | Serine / Threonine | Km bin./1mM Km bin. | 05-115 |
| TEC | Cytoplasmic Tyrosine | Km bin./1mM | 08-182 |
| TIE2(TEK) | Receptor Tyrosine | Km bin./1mM | 08-185 |
| TRKB(NTRK2) | Receptor Tyrosine | Km bin./1mM | 08-187 |
| TRKC(NTRK3) | Receptor Tyrosine | Km bin./1mM | 08-197 |
| TSSK1B(TSSK1) | Serine / Threonine | Km bin./1mM | 02-364 |
| TYRO3 | Receptor Tyrosine | Km bin./1mM | 08-109 |
| YES(YES1) | Cytoplasmic Tyrosine | Km bin./1mM | 08-175 |
No products matched.
Preincubation IC50 Determination Methodology
Reference Data
Service Schedule
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Test sample cutoff for next assays
Assay Start Day for MSA / IMAPTM
Assay Start Day for ADP-Glo™ and MSA(Preincubation)
Date in Red: Japan Holidays
Detailed Assay Conditions
for Each Kinase
Check this out & more in our Kinase Profiling Book!
Guide for Service Customers
