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- 生化学（セルフリー）プロファイリング/スクリーニング アッセイ受託サービス
- Mobility Shift Assay(MSA)/IMAP™ キナーゼアッセイ 受託サービス
- Mobility Shift Assay(MSA)/IMAP™ キナーゼアッセイ Preincubation試験ターゲット
Mobility Shift Assay(MSA)/IMAP™ キナーゼアッセイ Preincubation試験ターゲット
強い阻害効果を示すキナーゼ阻害剤の中には、キナーゼへの結合が非常に遅いもの（Slow binder)もあることが知られています。Preincubation IC50値算出試験では、Slow binderに適切な実験手順を用い化合物本来の阻害活性を測定致します。室温での活性の安定性が確認されたキナーゼのみをターゲットとし、通常のMobility Shift Assayの試験条件に室温 30 分のキナーゼ＋阻害剤での事前インキュベーション時間を加えて試験を行ないます。ATP濃度はKm値付近もしくは1mMからお選びください。
試験スケジュールおよびアッセイ詳細条件をご確認頂き、ご利用の際はPreincubation試験専用の申込書上でご希望ターゲットをご選択ください。ご記入済みの申込書はinfo@carnabio.com宛のメールに添付頂きご提出ください。
全キナーゼターゲット、セレクトパネルから選択をご希望の場合はこちらのページからお進みください。ご質問、ご要望などはお気軽にお問い合わせください。
| Target Name | Group | ATP conc. |
Source (Carna Cat. No.) |
| ABL(ABL1) | Cytoplasmic Tyrosine | Km bin./1mM | 08-001 |
| ABL(ABL1)[E255K] | Cytoplasmic Tyrosine | Km bin./1mM | 08-094 |
| ABL(ABL1)[T315I] | Cytoplasmic Tyrosine | Km bin./1mM | 08-093 |
| AKT1 | Serine / Threonine | Km bin./1mM | 01-101 |
| AKT2 | Serine / Threonine | Km bin. | 01-102 |
| AKT3 | Serine / Threonine | Km bin. | 01-103 |
| ALK | Receptor Tyrosine | Km bin./1mM | 08-518 |
| ALK[C1156Y] | Receptor Tyrosine | Km bin./1mM | 08-530 |
| ALK[F1174L] | Receptor Tyrosine | Km bin./1mM | 08-519 |
| ALK[G1202R] | Receptor Tyrosine | Km bin./1mM | 08-544 |
| ALK[G1269A] | Receptor Tyrosine | Km bin./1mM | 08-537 |
| ALK[L1196M] | Receptor Tyrosine | Km bin./1mM | 08-529 |
| ALK[R1275Q] | Receptor Tyrosine | Km bin./1mM | 08-520 |
| AMPKα1/β1/γ1 (PRKAA1/B1/G1) | Serine / Threonine | Km bin./1mM | 02-113 |
| AMPKα2/β1/γ1 | Serine / Threonine | Km bin. | 02-114 |
| ARG(ABL2) | Cytoplasmic Tyrosine | Km bin./1mM | 08-102 |
| AurA(AURKA)/TPX2 | Serine / Threonine | Km bin. | 05-101 |
| AurB(AURKB)／INCENP | Serine / Threonine | Km bin./1mM | 05-102 |
| BMX | Cytoplasmic Tyrosine | Km bin./1mM | 08-179 |
| BRSK1 | Serine / Threonine | Km bin./1mM | 02-115 |
| BRSK2 | Serine / Threonine | Km bin. | 02-116 |
| BUB1／BUB3 | Serine / Threonine | Km bin. | 05-187 |
| CaMK2α(CAMK2A) | Serine / Threonine | Km bin./1mM Km bin. | 02-109 |
| CaMK2β(CAMK2B) | Serine / Threonine | Km bin./1mM | 02-110 |
| CaMK2γ(CAMK2G) | Serine / Threonine | Km bin./1mM Km bin. | 02-112 |
| CaMK2δ(CAMK2D) | Serine / Threonine | Km bin./1mM Km bin. | 02-111 |
| CaMK4 | Serine / Threonine | Km bin./1mM | 02-108 |
| CDK1(CDC2)／CycB1 | Serine / Threonine | Km bin./1mM | 04-102 |
| CDK2／CycA2 | Serine / Threonine | Km bin./1mM | 04-103 |
| CDK2／CycE1 | Serine / Threonine | Km bin./1mM | 04-165 |
| CDK3／CycE1 | Serine / Threonine | Km bin. | 04-104 |
| CDK5／p25 | Serine / Threonine | Km bin./1mM | 04-106 |
| CGK2 | Serine / Threonine | Km bin. | 01-143 |
| CHK1(CHEK1) | Serine / Threonine | Km bin./1mM | 02-117 |
| CHK2(CHEK2) | Serine / Threonine | Km bin./1mM | 02-162 |
| CK1γ1 | Serine / Threonine | Km bin. | 03-105 |
| CK1γ2 | Serine / Threonine | Km bin. | 03-106 |
| CK1γ3 | Serine / Threonine | Km bin. | 03-107 |
| CK1δ | Serine / Threonine | Km bin./1mM | 03-103 |
| CK2α1/β (CSNK2A1) | Serine / Threonine | Km bin./1mM | 05-184 |
| CK2α2/β | Serine / Threonine | Km bin. | 05-185 |
| CLK1 | Serine / Threonine | Km bin./1mM | 04-126 |
| CLK2 | Serine / Threonine | Km bin./1mM | 04-127 |
| CLK3 | Serine / Threonine | Km bin. | 04-128 |
| DYRK1A | Serine / Threonine | Km bin./1mM | 04-130 |
| DYRK1B | Serine / Threonine | Km bin./1mM | 04-131 |
| DYRK2 | Serine / Threonine | Km bin./1mM | 04-132 |
| DYRK3 | Serine / Threonine | Km bin./1mM | 04-133 |
| EGFR (ERBB1) | Receptor Tyrosine | Km bin./1mM | 08-115 |
| EGFR[d746-750] (ERBB1) | Receptor Tyrosine | Km bin./1mM | 08-527 |
| EGFR[d746-750／C797S] NEW | Receptor Tyrosine | Km bin./1mM | 08-564 |
| EGFR[L858R] (ERBB1) | Receptor Tyrosine | Km bin./1mM | 08-502 |
| EGFR[L861Q] (ERBB1) | Receptor Tyrosine | Km bin./1mM | 08-513 |
| EGFR[T790M] (ERBB1) | Receptor Tyrosine | Km bin./1mM | 08-194 |
| EGFR[T790M／L858R] (ERBB1) | Receptor Tyrosine | Km bin./1mM | 08-510 |
| EPHA1 | Receptor Tyrosine | Km bin./1mM | 08-119 |
| EPHA2 | Receptor Tyrosine | Km bin./1mM | 08-121 |
| EPHA3 | Receptor Tyrosine | Km bin./1mM | 08-122 |
| EPHA4 | Receptor Tyrosine | Km bin./1mM | 08-123 |
| EPHA5 | Receptor Tyrosine | Km bin./1mM | 08-124 |
| EPHA6 | Receptor Tyrosine | Km bin./1mM | 08-125 |
| EPHA7 | Receptor Tyrosine | Km bin./1mM | 08-126 |
| EPHA8 | Receptor Tyrosine | Km bin./1mM | 08-127 |
| EPHB1 | Receptor Tyrosine | Km bin./1mM | 08-128 |
| EPHB2 | Receptor Tyrosine | Km bin./1mM | 08-129 |
| EPHB3 | Receptor Tyrosine | Km bin./1mM | 08-130 |
| EPHB4 | Receptor Tyrosine | Km bin./1mM | 08-131 |
| Erk1(MAPK3) | Serine / Threonine | Km bin./1mM | 04-142 |
| Erk2(MAPK1) | Serine / Threonine | Km bin./1mM | 04-143 |
| FER | Cytoplasmic Tyrosine | Km bin./1mM | 08-139 |
| FES | Cytoplasmic Tyrosine | Km bin./1mM | 08-140 |
| FGFR1 | Receptor Tyrosine | Km bin./1mM | 08-133 |
| FGFR2 | Receptor Tyrosine | Km bin./1mM | 08-134 |
| FGFR2[V564I] | Receptor Tyrosine | Km bin./1mM | 08-546 |
| FGFR4 | Receptor Tyrosine | Km bin./1mM | 08-136 |
| FGFR4[V550L] | Receptor Tyrosine | Km bin./1mM | 08-526 |
| FLT1 | Receptor Tyrosine | Km bin./1mM | 08-189 |
| FLT4 | Receptor Tyrosine | Km bin./1mM | 08-190 |
| FRK | Cytoplasmic Tyrosine | Km bin./1mM | 08-167 |
| GSK3α | Serine / Threonine | Km bin./1mM | 04-140 |
| GSK3β(GSK3B) | Serine / Threonine | Km bin./1mM | 04-141 |
| Haspin | Serine / Threonine | Km bin. | 05-111 |
| HER4 (ERBB4) | Receptor Tyrosine | Km bin./1mM | 08-118 |
| HIPK1 | Serine / Threonine | Km bin. | 04-135 |
| HIPK2 | Serine / Threonine | Km bin. | 04-136 |
| HIPK3 | Serine / Threonine | Km bin./1mM | 04-137 |
| IGF1R | Receptor Tyrosine | Km bin./1mM | 08-141 |
| IKKβ (IKBKB) | Serine / Threonine | Km bin./1mM | 05-084 |
| INSR | Receptor Tyrosine | Km bin./1mM | 08-142 |
| JAK2 | Cytoplasmic Tyrosine | Km bin./1mM | 08-045 |
| JNK1(MAPK8) | Serine / Threonine | Km bin./1mM | 04-163 |
| JNK2(MAPK9) | Serine / Threonine | Km bin./1mM | 04-164 |
| JNK3 (MAPK10) | Serine / Threonine | Km bin./1mM | 04-150 |
| KDR(VEGFR2) | Receptor Tyrosine | Km bin./1mM | 08-191 |
| LCK | Cytoplasmic Tyrosine | Km bin./1mM | 08-170 |
| LYNa | Cytoplasmic Tyrosine | Km bin./1mM | 08-171 |
| LYNb | Cytoplasmic Tyrosine | Km bin./1mM | 08-172 |
| MAPKAPK2 | Serine / Threonine | Km bin./1mM | 02-142 |
| MAPKAPK3 | Serine / Threonine | Km bin. | 02-143 |
| MAPKAPK5 | Serine / Threonine | Km bin. | 02-144 |
| MARK1 | Serine / Threonine | Km bin. | 02-120 |
| MARK2 | Serine / Threonine | Km bin. | 02-121 |
| MARK3 | Serine / Threonine | Km bin. | 02-122 |
| MARK4 | Serine / Threonine | Km bin./1mM | 02-123 |
| MER(MERTK) | Receptor Tyrosine | Km bin./1mM | 08-108 |
| MET | Receptor Tyrosine | Km bin./1mM | 08-151 |
| MET[D1228H] | Receptor Tyrosine | Km bin./1mM | 08-540 |
| MET[M1250T] | Receptor Tyrosine | Km bin./1mM | 08-545 |
| MET[Y1235D] | Receptor Tyrosine | Km bin./1mM | 08-198 |
| MNK1(MKNK1) | Serine / Threonine | Km bin. | 02-145 |
| MNK2 | Serine / Threonine | Km bin./1mM | 02-146 |
| MSK1 | Serine / Threonine | Km bin./1mM Km bin. | 01-147 |
| NEK2 | Serine / Threonine | Km bin./1mM | 05-226 |
| NEK4 | Serine / Threonine | Km bin. | 05-128 |
| NIM1K(MGC42105) | Serine / Threonine | Km bin. | 02-175 |
| NPM1-ALK | Receptor Tyrosine | Km bin./1mM | 08-517 |
| NuaK1(ARK5) | Serine / Threonine | Km bin./1mM | 02-126 |
| NuaK2 | Serine / Threonine | Km bin./1mM | 02-127 |
| p38α(MAPK14) | Serine / Threonine | Km bin./1mM | 04-152 |
| p38β (MAPK11) | Serine / Threonine | Km bin./1mM | 04-153 |
| p38γ (MAPK12) | Serine / Threonine | Km bin./1mM | 04-155 |
| p38δ (MAPK13) | Serine / Threonine | Km bin./1mM | 04-154 |
| p70S6K(RPS6KB1) | Serine / Threonine | Km bin./1mM | 01-154 |
| p70S6Kβ(RPS6KB2) | Serine / Threonine | Km bin. | 01-155 |
| PAK1 | Serine / Threonine | Km bin./1mM | 07-123 |
| PAK2 | Serine / Threonine | Km bin./1mM | 07-124 |
| PAK5 | Serine / Threonine | Km bin./1mM | 07-127 |
| PDGFRα(PDGFRA) | Receptor Tyrosine | Km bin./1mM | 08-157 |
| PDGFRα(PDGFRA)[D842V] | Receptor Tyrosine | Km bin./1mM | 08-506 |
| PDGFRα(PDGFRA)[V561D] | Receptor Tyrosine | Km bin./1mM | 08-507 |
| PDGFRβ(PDGFRB) | Receptor Tyrosine | Km bin./1mM | 08-158 |
| PIM1 | Serine / Threonine | Km bin./1mM | 02-054 |
| PKCε(PRKCE) | Serine / Threonine | Km bin./1mM | 01-136 |
| PKD1 | Serine / Threonine | Km bin. | 02-157 |
| PKD2 (PRKD2) | Serine / Threonine | Km bin./1mM | 02-158 |
| PYK2(PTK2B) | Cytoplasmic Tyrosine | Km bin./1mM | 08-138 |
| QIK (SIK2) | Serine / Threonine | Km bin./1mM | 02-129 |
| RET | Receptor Tyrosine | Km bin./1mM | 08-159 |
| RET[G691S] | Receptor Tyrosine | Km bin./1mM | 08-522 |
| RET[M918T] | Receptor Tyrosine | Km bin./1mM | 08-508 |
| RET[S891A] | Receptor Tyrosine | Km bin./1mM | 08-523 |
| RET[Y791F] | Receptor Tyrosine | Km bin./1mM | 08-521 |
| RON (MST1R) | Receptor Tyrosine | Km bin./1mM | 08-152 |
| ROS(ROS1) | Receptor Tyrosine | Km bin./1mM | 08-163 |
| RSK3(RPS6KA2) | Serine / Threonine | Km bin./1mM | 01-151 |
| RSK4(RPS6KA6) | Serine / Threonine | Km bin./1mM | 01-152 |
| SGK | Serine / Threonine | Km bin./1mM | 01-158 |
| SGK3 | Serine / Threonine | Km bin. | 01-160 |
| skMLCK | Serine / Threonine | Km bin. | 02-150 |
| SRC | Cytoplasmic Tyrosine | Km bin./1mM | 08-173 |
| SRM (SRMS) | Cytoplasmic Tyrosine | Km bin./1mM | 08-174 |
| TBK1 | Serine / Threonine | Km bin./1mM Km bin. | 05-115 |
| TEC | Cytoplasmic Tyrosine | Km bin./1mM | 08-182 |
| TIE2(TEK) | Receptor Tyrosine | Km bin./1mM | 08-185 |
| TRKB(NTRK2) | Receptor Tyrosine | Km bin./1mM | 08-187 |
| TRKC(NTRK3) | Receptor Tyrosine | Km bin./1mM | 08-197 |
| TSSK1 | Serine / Threonine | Km bin./1mM | 02-364 |
| TYRO3 | Receptor Tyrosine | Km bin./1mM | 08-109 |
| YES(YES1) | Cytoplasmic Tyrosine | Km bin./1mM | 08-175 |
| YES(YES1)[T348I] | Cytoplasmic Tyrosine | Km bin./1mM | 08-533 |
Preincubation IC50値算出試験
ご参考実験データ
アッセイスケジュール
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| 2024年10月 | ||||||
| 日 | 月 | 火 | 水 | 木 | 金 | 土 |
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| 1 | 2 | 3 | 4 | 5 | ||
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| 20 | 21 | 22 | 23 | 24 | 25 | 26 |
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被験物質 受付締切日
MSA / IMAPTMアッセイ開始日
ADP-Glo™, MSA(Preincubation)アッセイ開始日
各アッセイ詳細
Bookを開くと各アッセイの詳細条件がご覧いただけます。
